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Lipidomic profiling of human serum enables detection of pancreatic cancer

D. Wolrab, R. Jirásko, E. Cífková, M. Höring, D. Mei, M. Chocholoušková, O. Peterka, J. Idkowiak, T. Hrnčiarová, L. Kuchař, R. Ahrends, R. Brumarová, D. Friedecký, G. Vivo-Truyols, P. Škrha, J. Škrha, R. Kučera, B. Melichar, G. Liebisch, R....

. 2022 ; 13 (1) : 124. [pub] 20220110

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc22011362

Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve the survival time prognosis of patients, who are often diagnosed too late at an incurable stage. Several studies report the dysregulation of lipid metabolism in tumor cells, suggesting that changes in the blood lipidome may accompany tumor growth. Here we show that the comprehensive mass spectrometric determination of a wide range of serum lipids reveals statistically significant differences between pancreatic cancer patients and healthy controls, as visualized by multivariate data analysis. Three phases of biomarker discovery research (discovery, qualification, and verification) are applied for 830 samples in total, which shows the dysregulation of some very long chain sphingomyelins, ceramides, and (lyso)phosphatidylcholines. The sensitivity and specificity to diagnose pancreatic cancer are over 90%, which outperforms CA 19-9, especially at an early stage, and is comparable to established diagnostic imaging methods. Furthermore, selected lipid species indicate a potential as prognostic biomarkers.

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$a Wolrab, Denise $u Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic $1 https://orcid.org/0000000326643912
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$a Lipidomic profiling of human serum enables detection of pancreatic cancer / $c D. Wolrab, R. Jirásko, E. Cífková, M. Höring, D. Mei, M. Chocholoušková, O. Peterka, J. Idkowiak, T. Hrnčiarová, L. Kuchař, R. Ahrends, R. Brumarová, D. Friedecký, G. Vivo-Truyols, P. Škrha, J. Škrha, R. Kučera, B. Melichar, G. Liebisch, R. Burkhardt, MR. Wenk, A. Cazenave-Gassiot, P. Karásek, I. Novotný, K. Greplová, R. Hrstka, M. Holčapek
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$a Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve the survival time prognosis of patients, who are often diagnosed too late at an incurable stage. Several studies report the dysregulation of lipid metabolism in tumor cells, suggesting that changes in the blood lipidome may accompany tumor growth. Here we show that the comprehensive mass spectrometric determination of a wide range of serum lipids reveals statistically significant differences between pancreatic cancer patients and healthy controls, as visualized by multivariate data analysis. Three phases of biomarker discovery research (discovery, qualification, and verification) are applied for 830 samples in total, which shows the dysregulation of some very long chain sphingomyelins, ceramides, and (lyso)phosphatidylcholines. The sensitivity and specificity to diagnose pancreatic cancer are over 90%, which outperforms CA 19-9, especially at an early stage, and is comparable to established diagnostic imaging methods. Furthermore, selected lipid species indicate a potential as prognostic biomarkers.
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$a Jirásko, Robert $u Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic
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$a Ahrends, Robert $u Department of Analytical Chemistry, University of Vienna, Vienna, Austria
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$a Karásek, Petr $u Clinic of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic
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$a Greplová, Kristína $u Faculty of Medicine, Masaryk University, Brno, Czech Republic $u Research Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic
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