-
Je něco špatně v tomto záznamu ?
Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission
V. Kselíková, V. Zachleder, K. Bišová
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- buněčné dělení * MeSH
- buněčný cyklus * MeSH
- Viridiplantae MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This work represents a detailed guide for commitment point analysis in microalgae dividing by multiple fission. The method is based on allowing the committed cells to divide in favorable conditions in the dark. This protocol offers a strategy to monitor cell cycle progression, both in control cultures and cultures treated with compounds affecting cell cycle length and/or progression. As the variety of such compounds is wide, our aim was to make the protocol easily modifiable to various research aims. The technique is easy to follow, low-cost, does not require any special equipment and offers reliable results in a reasonable time. The protocol offers step-by-step instructions, explains the theory behind these steps and offers solutions to some of the problems that may arise during the procedure.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22011545
- 003
- CZ-PrNML
- 005
- 20220506131012.0
- 007
- ta
- 008
- 220425s2022 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-0716-1744-1_5 $2 doi
- 035 __
- $a (PubMed)34705234
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Kselíková, Veronika $u Centre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Třeboň, Czech Republic $u Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic
- 245 10
- $a Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission / $c V. Kselíková, V. Zachleder, K. Bišová
- 520 9_
- $a This work represents a detailed guide for commitment point analysis in microalgae dividing by multiple fission. The method is based on allowing the committed cells to divide in favorable conditions in the dark. This protocol offers a strategy to monitor cell cycle progression, both in control cultures and cultures treated with compounds affecting cell cycle length and/or progression. As the variety of such compounds is wide, our aim was to make the protocol easily modifiable to various research aims. The technique is easy to follow, low-cost, does not require any special equipment and offers reliable results in a reasonable time. The protocol offers step-by-step instructions, explains the theory behind these steps and offers solutions to some of the problems that may arise during the procedure.
- 650 12
- $a buněčný cyklus $7 D002453
- 650 12
- $a buněčné dělení $7 D002455
- 650 _2
- $a Viridiplantae $7 D057948
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Zachleder, Vilém $u Centre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Třeboň, Czech Republic
- 700 1_
- $a Bišová, Kateřina $u Centre Algatech, Laboratory of Cell Cycles of Algae, Institute of Microbiology of the Czech Academy of Sciences, Třeboň, Czech Republic. bisova@alga.cz
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 2382, č. - (2022), s. 89-101
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/34705234 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20220425 $b ABA008
- 991 __
- $a 20220506131004 $b ABA008
- 999 __
- $a ok $b bmc $g 1789251 $s 1162743
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2022 $b 2382 $c - $d 89-101 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20220425
