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Investigation of ochratoxin A biomarkers in biological materials obtained from patients suffering from renal cell carcinoma

F. Malir, M. Louda, J. Toman, V. Ostry, D. Pickova, J. Pacovsky, M. Brodak, A. Pfohl-Leszkowicz

. 2021 ; 158 (-) : 112669. [pub] 20211112

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22011811

Ochratoxin A (OTA) exposure can result in chronic renal diseases and cancer. The incidence of kidney, renal pelvis, and ureter malignant neoplasms in the Czech Republic is approximately 29.5 renal tumours per 100,000 inhabitants. The question arises whether mycotoxins are also involved in kidney disease and cancer. A sensitive validated analytical methodology, based on an immunoaffinity clean-up followed by HPLC with fluorescence detection, was developed to explore whether OTA accumulates in clear renal cell carcinoma-adenocarcinoma in Czech patients. Simultaneously, DNA-adducts and OTA metabolites were qualitatively analysed in tissues and urine. OTA was analysed in 33 kidney and tumour samples from 26 men and 7 women collected during nephrectomy from patients of the East Bohemian region from 2015 to 2017. OTA was found in 76% of the analysed samples. Its concentrations ranged from not detectable to 390 ng/kg with a median of 167 ng/kg in kidney samples and from not detectable to 430 ng/kg with a median of 122 ng/kg in tumour samples. Urinary OTA metabolites and DNA adducts were qualitatively analysed for the corresponding 20 patients. The presence of some OTA metabolites such as ochratoxin A hydroquinone and/or decarboxylated ochratoxin A hydroquinone correlate with the presence of OTA-DNA adducts.

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$a Louda, Miroslav $u Charles University Medical School and Teaching Hospital, Department of Urology, Hradec Kralove, Czech Republic
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$a Ostry, Vladimir $u University of Hradec Kralove, Faculty of Science, Department of Biology, Hradec Kralove, Czech Republic; National Institute of Public Health in Prague, Center for Health, Nutrition and Food, Brno, Czech Republic
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$a Pickova, Darina $u University of Hradec Kralove, Faculty of Science, Department of Biology, Hradec Kralove, Czech Republic
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