Ochratoxin A (OTA) exposure can result in chronic renal diseases and cancer. The incidence of kidney, renal pelvis, and ureter malignant neoplasms in the Czech Republic is approximately 29.5 renal tumours per 100,000 inhabitants. The question arises whether mycotoxins are also involved in kidney disease and cancer. A sensitive validated analytical methodology, based on an immunoaffinity clean-up followed by HPLC with fluorescence detection, was developed to explore whether OTA accumulates in clear renal cell carcinoma-adenocarcinoma in Czech patients. Simultaneously, DNA-adducts and OTA metabolites were qualitatively analysed in tissues and urine. OTA was analysed in 33 kidney and tumour samples from 26 men and 7 women collected during nephrectomy from patients of the East Bohemian region from 2015 to 2017. OTA was found in 76% of the analysed samples. Its concentrations ranged from not detectable to 390 ng/kg with a median of 167 ng/kg in kidney samples and from not detectable to 430 ng/kg with a median of 122 ng/kg in tumour samples. Urinary OTA metabolites and DNA adducts were qualitatively analysed for the corresponding 20 patients. The presence of some OTA metabolites such as ochratoxin A hydroquinone and/or decarboxylated ochratoxin A hydroquinone correlate with the presence of OTA-DNA adducts.
- MeSH
- adukty DNA MeSH
- karcinom z renálních buněk * metabolismus patologie MeSH
- ledviny metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery analýza MeSH
- nádory ledvin * metabolismus patologie MeSH
- ochratoxiny analýza MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Transgenic Bt maize is a potentially important tool against insect pest in the EU and other countries. Bt maize (e.g. MON 810, Bt 11) which carries the Bt gene is highly resistant to larval feeding of European corn borer, stalk borer, and Southwestern corn borer, depending on Bt toxin (δ toxin) production. Effective measures used to fight pests may often have positive side-effects in that they may also contribute to reducing mycotoxin concentrations. A systematic review has been used for the purposes of evaluating the studies on the reduction of aflatoxins in Bt maize. According to five studies, Bt maize has significantly lower concentrations of aflatoxins than non-Bt maize hybrids, only one study has shown no significant effect of Bt maize. Other studies have shown mixed results (four studies). The results of these studies were influenced by the year of sampling or by using maize breeding lines selected for resistance to aflatoxin accumulation.
- Klíčová slova
- Bt kukuřice,
- MeSH
- aflatoxiny * škodlivé účinky MeSH
- bezpečnost potravin MeSH
- geneticky modifikované rostliny * MeSH
- kukuřice setá * genetika MeSH
- mykotoxiny MeSH
- odolnost vůči nemocem genetika MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
