Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome

S. Hankeova, J. Salplachta, T. Zikmund, M. Kavkova, N. Van Hul, A. Brinek, V. Smekalova, J. Laznovsky, F. Dawit, J. Jaros, V. Bryja, U. Lendahl, E. Ellis, A. Nemeth, B. Fischler, E. Hannezo, J. Kaiser, ER. Andersson

. 2021 ; 10 (-) : . [pub] 20210226

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22012524

Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22012524
003      
CZ-PrNML
005      
20250514090533.0
007      
ta
008      
220425e20210226xxk f 000 0|eng||
009      
AR
024    7_
$a 10.7554/eLife.60916 $2 doi
035    __
$a (PubMed)33635272
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Hankeova, Simona $u Department of Biosciences and Nutrition, Karolinska Institutet, Solna, Sweden $u Department of Experimental Biology, Masaryk University, Brno, Czech Republic $1 https://orcid.org/0000000277973818
245    10
$a DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome / $c S. Hankeova, J. Salplachta, T. Zikmund, M. Kavkova, N. Van Hul, A. Brinek, V. Smekalova, J. Laznovsky, F. Dawit, J. Jaros, V. Bryja, U. Lendahl, E. Ellis, A. Nemeth, B. Fischler, E. Hannezo, J. Kaiser, ER. Andersson
520    9_
$a Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.
650    _2
$a Alagillův syndrom $x patofyziologie $7 D016738
650    _2
$a zvířata $7 D000818
650    _2
$a žlučové cesty $x růst a vývoj $x patofyziologie $7 D001652
650    _2
$a modely nemocí na zvířatech $7 D004195
650    _2
$a myši $7 D051379
650    _2
$a myši transgenní $7 D008822
650    _2
$a rentgenová mikrotomografie $x klasifikace $x metody $7 D055114
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Salplachta, Jakub $u CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic $1 https://orcid.org/0000000201497843
700    1_
$a Zikmund, Tomas $u CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
700    1_
$a Kavkova, Michaela $u CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
700    1_
$a Van Hul, Noémi $u Department of Biosciences and Nutrition, Karolinska Institutet, Solna, Sweden $1 https://orcid.org/0000000314108808
700    1_
$a Brinek, Adam $u CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
700    1_
$a Smekalova, Veronika $u CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
700    1_
$a Lázňovský, Jakub $u CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic $7 xx0331997
700    1_
$a Dawit, Feven $u Department of Pediatrics, Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet and Karolinska University Hospital, Solna, Sweden
700    1_
$a Jaros, Josef $u Department of Histology and Embryology, Masaryk University, Brno, Czech Republic
700    1_
$a Bryja, Vítězslav $u Department of Experimental Biology, Masaryk University, Brno, Czech Republic $1 https://orcid.org/0000000291365085 $7 xx0035073
700    1_
$a Lendahl, Urban $u Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden
700    1_
$a Ellis, Ewa $u Department of Pediatrics, Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet and Karolinska University Hospital, Solna, Sweden $1 https://orcid.org/0000000230575337
700    1_
$a Nemeth, Antal $u Department of Laboratory Medicine, Karolinska Institutet, Solna, Sweden
700    1_
$a Fischler, Björn $u Department of Pediatrics, Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet and Karolinska University Hospital, Solna, Sweden
700    1_
$a Hannezo, Edouard $u Institute of Science and Technology, Klosterneuburg, Austria $1 https://orcid.org/0000000160051561
700    1_
$a Kaiser, Jozef $u CEITEC - Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic
700    1_
$a Andersson, Emma Rachel $u Department of Biosciences and Nutrition, Karolinska Institutet, Solna, Sweden $u Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden $1 https://orcid.org/000000028608625X
773    0_
$w MED00188753 $t eLife $x 2050-084X $g Roč. 10 (20210226)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33635272 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20250514090535 $b ABA008
999    __
$a ok $b bmc $g 1789926 $s 1163725
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 10 $c - $e 20210226 $i 2050-084X $m eLife $n eLife $x MED00188753
LZP    __
$a Pubmed-20220425

Najít záznam

Citační ukazatele

Možnosti archivace