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Recommendations for good practice in MS-based lipidomics

HC. Köfeler, R. Ahrends, ES. Baker, K. Ekroos, X. Han, N. Hoffmann, M. Holčapek, MR. Wenk, G. Liebisch

. 2021 ; 62 (-) : 100138. [pub] 20211016

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem, přehledy

Perzistentní odkaz   https://www.medvik.cz/link/bmc22012568

Grantová podpora
P30 ES025128 NIEHS NIH HHS - United States
P42 ES027704 NIEHS NIH HHS - United States
RF1 AG061872 NIA NIH HHS - United States
P42 ES031009 NIEHS NIH HHS - United States
RF1 AG061729 NIA NIH HHS - United States

In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field.

Citace poskytuje Crossref.org

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