In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field.

Barshop Inst Longev and Aging Studies Univ Texas Hlth Sci Ctr San Antonio San Antonio TX USA

Center for Biotechnology Universität Bielefeld Bielefeld Germany

Core Facility Mass Spectrometry Medical University of Graz Graz Austria

Department for Analytical Chemistry University of Vienna Vienna Austria

Department of Analytical Chemistry Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic

Department of Chemistry North Carolina State University Raleigh NC USA

Institute of Clinical Chemistry and Laboratory Medicine Regensburg University Hospital Regensburg Germany

Lipidomics Consulting Ltd Esbo Finland

Singapore Lipidomics Incubator Department of Biochemistry YLL School of Medicine National University of Singapore Singapore Singapore

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