Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

ImmunoDisk-A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored

B. Johannsen, D. Baumgartner, L. Karkossa, N. Paust, M. Karpíšek, N. Bostanci, R. Zengerle, K. Mitsakakis

. 2022 ; 12 (6) : . [pub] 20220614

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22018005

Grantová podpora
633780 European Commission
E!113595 Eurostars, managed by the Federal Ministry of Education & Research (BMBF)

In this paper, we present the ImmunoDisk, a fully automated sample-to-answer centrifugal microfluidic cartridge, integrating a heterogeneous, wash-free, magnetic- and fluorescent bead-based immunoassay (bound-free phase detection immunoassay/BFPD-IA). The BFPD-IA allows the implementation of a simple fluidic structure, where the assay incubation, bead separation and detection are performed in the same chamber. The system was characterized using a C-reactive protein (CRP) competitive immunoassay. A parametric investigation on air drying of protein-coupled beads for pre-storage at room temperature is presented. The key parameters were buffer composition, drying temperature and duration. A protocol for drying two different types of protein-coupled beads with the same temperature and duration using different drying buffers is presented. The sample-to-answer workflow was demonstrated measuring CRP in 5 μL of human serum, without prior dilution, utilizing only one incubation step, in 20 min turnaround time, in the clinically relevant concentration range of 15-115 mg/L. A reproducibility assessment over three disk batches revealed an average signal coefficient of variation (CV) of 5.8 ± 1.3%. A CRP certified reference material was used for method verification with a concentration CV of 8.6%. Our results encourage future testing of the CRP-ImmunoDisk in clinical studies and its point-of-care implementation in many diagnostic applications.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22018005
003      
CZ-PrNML
005      
20220804134513.0
007      
ta
008      
220720s2022 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/bios12060413 $2 doi
035    __
$a (PubMed)35735560
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Johannsen, Benita $u Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
245    10
$a ImmunoDisk-A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored / $c B. Johannsen, D. Baumgartner, L. Karkossa, N. Paust, M. Karpíšek, N. Bostanci, R. Zengerle, K. Mitsakakis
520    9_
$a In this paper, we present the ImmunoDisk, a fully automated sample-to-answer centrifugal microfluidic cartridge, integrating a heterogeneous, wash-free, magnetic- and fluorescent bead-based immunoassay (bound-free phase detection immunoassay/BFPD-IA). The BFPD-IA allows the implementation of a simple fluidic structure, where the assay incubation, bead separation and detection are performed in the same chamber. The system was characterized using a C-reactive protein (CRP) competitive immunoassay. A parametric investigation on air drying of protein-coupled beads for pre-storage at room temperature is presented. The key parameters were buffer composition, drying temperature and duration. A protocol for drying two different types of protein-coupled beads with the same temperature and duration using different drying buffers is presented. The sample-to-answer workflow was demonstrated measuring CRP in 5 μL of human serum, without prior dilution, utilizing only one incubation step, in 20 min turnaround time, in the clinically relevant concentration range of 15-115 mg/L. A reproducibility assessment over three disk batches revealed an average signal coefficient of variation (CV) of 5.8 ± 1.3%. A CRP certified reference material was used for method verification with a concentration CV of 8.6%. Our results encourage future testing of the CRP-ImmunoDisk in clinical studies and its point-of-care implementation in many diagnostic applications.
650    12
$a C-reaktivní protein $7 D002097
650    _2
$a lidé $7 D006801
650    _2
$a imunoanalýza $x metody $7 D007118
650    _2
$a indikátory a reagencie $7 D007202
650    12
$a mikrofluidika $7 D044085
650    _2
$a reprodukovatelnost výsledků $7 D015203
655    _2
$a časopisecké články $7 D016428
700    1_
$a Baumgartner, Desirée $u Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
700    1_
$a Karkossa, Lena $u Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
700    1_
$a Paust, Nils $u Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany $u Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
700    1_
$a Karpíšek, Michal $u BioVendor-Laboratorní Medicína a.s., Research & Diagnostic Products Division, Karasek 1767/1, Reckovice, 62100 Brno, Czech Republic $u Faculty of Pharmacy, Masaryk University, Palackeho trida 1946/1, 61242 Brno, Czech Republic $1 https://orcid.org/0000000165034250
700    1_
$a Bostanci, Nagihan $u Section of Oral Health and Periodontology, Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, 14104 Huddinge, Sweden
700    1_
$a Zengerle, Roland $u Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany $u Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
700    1_
$a Mitsakakis, Konstantinos $u Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany $u Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany $1 https://orcid.org/0000000232879123
773    0_
$w MED00197236 $t Biosensors $x 2079-6374 $g Roč. 12, č. 6 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35735560 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804134507 $b ABA008
999    __
$a ok $b bmc $g 1821874 $s 1169248
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 12 $c 6 $e 20220614 $i 2079-6374 $m Biosensors $n Biosensors (Basel) $x MED00197236
GRA    __
$a 633780 $p European Commission
GRA    __
$a E!113595 $p Eurostars, managed by the Federal Ministry of Education & Research (BMBF)
LZP    __
$a Pubmed-20220720

Najít záznam

Citační ukazatele

Možnosti archivace