-
Je něco špatně v tomto záznamu ?
Biogenesis of hepatitis B virus e antigen is driven by translocon-associated protein complex and regulated by conserved cysteine residues within its signal peptide sequence
H. Zábranská, A. Zábranský, B. Lubyová, J. Hodek, A. Křenková, M. Hubálek, J. Weber, I. Pichová
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 2005 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2005-01-01 do Před 1 rokem
Wiley Free Content
od 2005 do Před 1 rokem
PubMed
34839586
DOI
10.1111/febs.16304
Knihovny.cz E-zdroje
- MeSH
- cystein metabolismus MeSH
- endoplazmatické retikulum metabolismus MeSH
- hepatitida B - antigeny e * metabolismus MeSH
- hepatitida B * metabolismus MeSH
- lidé MeSH
- membránové glykoproteiny MeSH
- proteiny - lokalizační signály genetika MeSH
- proteiny vázající vápník MeSH
- receptory cytoplazmatické a nukleární MeSH
- receptory peptidů MeSH
- virus hepatitidy B metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Hepatitis B virus uses e antigen (HBe), which is dispensable for virus infectivity, to modulate host immune responses and achieve viral persistence in human hepatocytes. The HBe precursor (p25) is directed to the endoplasmic reticulum (ER), where cleavage of the signal peptide (sp) gives rise to the first processing product, p22. P22 can be retro-translocated back to the cytosol or enter the secretory pathway and undergo a second cleavage event, resulting in secreted p17 (HBe). Here, we report that translocation of p25 to the ER is promoted by translocon-associated protein complex. We have found that p25 is not completely translocated into the ER; a fraction of p25 is phosphorylated and remains in the cytoplasm and nucleus. Within the p25 sp sequence, we have identified three cysteine residues that control the efficiency of sp cleavage and contribute to proper subcellular distribution of the precore pool.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22018558
- 003
- CZ-PrNML
- 005
- 20220804134847.0
- 007
- ta
- 008
- 220720s2022 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1111/febs.16304 $2 doi
- 035 __
- $a (PubMed)34839586
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Zábranská, Helena $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
- 245 10
- $a Biogenesis of hepatitis B virus e antigen is driven by translocon-associated protein complex and regulated by conserved cysteine residues within its signal peptide sequence / $c H. Zábranská, A. Zábranský, B. Lubyová, J. Hodek, A. Křenková, M. Hubálek, J. Weber, I. Pichová
- 520 9_
- $a Hepatitis B virus uses e antigen (HBe), which is dispensable for virus infectivity, to modulate host immune responses and achieve viral persistence in human hepatocytes. The HBe precursor (p25) is directed to the endoplasmic reticulum (ER), where cleavage of the signal peptide (sp) gives rise to the first processing product, p22. P22 can be retro-translocated back to the cytosol or enter the secretory pathway and undergo a second cleavage event, resulting in secreted p17 (HBe). Here, we report that translocation of p25 to the ER is promoted by translocon-associated protein complex. We have found that p25 is not completely translocated into the ER; a fraction of p25 is phosphorylated and remains in the cytoplasm and nucleus. Within the p25 sp sequence, we have identified three cysteine residues that control the efficiency of sp cleavage and contribute to proper subcellular distribution of the precore pool.
- 650 _2
- $a proteiny vázající vápník $7 D002135
- 650 _2
- $a cystein $x metabolismus $7 D003545
- 650 _2
- $a endoplazmatické retikulum $x metabolismus $7 D004721
- 650 12
- $a hepatitida B $x metabolismus $7 D006509
- 650 12
- $a hepatitida B - antigeny e $x metabolismus $7 D006513
- 650 _2
- $a virus hepatitidy B $x metabolismus $7 D006515
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a membránové glykoproteiny $7 D008562
- 650 _2
- $a proteiny - lokalizační signály $x genetika $7 D021382
- 650 _2
- $a receptory cytoplazmatické a nukleární $7 D018160
- 650 _2
- $a receptory peptidů $7 D018000
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Zábranský, Aleš $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Lubyová, Barbora $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Hodek, Jan $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Křenková, Alena $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Hubálek, Martin $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Weber, Jan $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
- 700 1_
- $a Pichová, Iva $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic $1 https://orcid.org/0000000221789819 $7 xx0101840
- 773 0_
- $w MED00008414 $t The FEBS journal $x 1742-4658 $g Roč. 289, č. 10 (2022), s. 2895-2914
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/34839586 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20220720 $b ABA008
- 991 __
- $a 20220804134841 $b ABA008
- 999 __
- $a ok $b bmc $g 1822248 $s 1169801
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2022 $b 289 $c 10 $d 2895-2914 $e 20211218 $i 1742-4658 $m The FEBS journal $n FEBS J $x MED00008414
- LZP __
- $a Pubmed-20220720