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Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E
SI. Dewees, R. Vargová, KR. Hardin, RE. Turn, S. Devi, J. Linnert, U. Wolfrum, T. Caspary, M. Eliáš, RA. Kahn
Language English Country United States
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
F31 HD096815
NICHD NIH HHS - United States
R35 GM122549
NIGMS NIH HHS - United States
R35 GM122568
NIGMS NIH HHS - United States
NLK
Free Medical Journals
from 1992 to 2 months ago
PubMed Central
from 1992 to 2 months ago
Europe PubMed Central
from 1992 to 2 months ago
Open Access Digital Library
from 1989-11-01
Open Access Digital Library
from 1997-01-01
- MeSH
- Cilia metabolism MeSH
- Fibroblasts * metabolism MeSH
- Phosphoric Monoester Hydrolases * metabolism MeSH
- Phylogeny MeSH
- Mice MeSH
- Proteins metabolism MeSH
- Protein Transport MeSH
- Carrier Proteins genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
The ARF family of regulatory GTPases is ancient, with 16 members predicted to have been present in the last eukaryotic common ancestor. Our phylogenetic profiling of paralogues in diverse species identified four family members whose presence correlates with that of a cilium/flagellum: ARL3, ARL6, ARL13, and ARL16. No prior evidence links ARL16 to cilia or other cell functions, despite its presence throughout eukaryotes. Deletion of ARL16 in mouse embryonic fibroblasts (MEFs) results in decreased ciliogenesis yet increased ciliary length. We also found Arl16 knockout (KO) in MEFs to alter ciliary protein content, including loss of ARL13B, ARL3, INPP5E, and the IFT-A core component IFT140. Instead, both INPP5E and IFT140 accumulate at the Golgi in Arl16 KO lines, while other intraflagellar transport (IFT) proteins do not, suggesting a specific defect in traffic from Golgi to cilia. We propose that ARL16 regulates a Golgi-cilia traffic pathway and is required specifically in the export of IFT140 and INPP5E from the Golgi.
Department of Biochemistry Emory University School of Medicine Atlanta GA 30322
Department of Cell Biology Emory University School of Medicine Atlanta GA 30322
Department of Human Genetics Emory University School of Medicine Atlanta GA 30322
Department of Microbiology and Immunology Stanford University Palo Alto CA 94305 5124
Institute of Molecular Physiology Johannes Gutenberg University Mainz 55128 Germany
References provided by Crossref.org
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