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Expression and 7-day time course of circulating microRNAs in septic patients treated with nephrotoxic antibiotic agents

N. Petejova, A. Martinek, J. Zadrazil, V. Klementa, L. Pribylova, R. Bris, M. Kanova, R. Sigutova, I. Kacirova, Z. Svagera, E. Bace, D. Stejskal

. 2022 ; 23 (1) : 111. [pub] 20220319

Language English Country Great Britain

Document type Clinical Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc22019151

BACKGROUND: Through regulation of signaling pathways, microRNAs (miRNAs) can be involved in sepsis and associated organ dysfunction. The aims of this study were to track the 7-day time course of blood miRNAs in patients with sepsis treated with vancomycin, gentamicin, or a non-nephrotoxic antibiotic and miRNA associations with neutrophil gelatinase-associated lipokalin (NGAL), creatinine, procalcitonin, interleukin-6, and acute kidney injury (AKI) stage. METHODS: Of 46 adult patients, 7 were on vancomycin, 20 on gentamicin, and 19 on another antibiotic. Blood samples were collected on days 1, 4, and 7 of treatment, and miRNAs were identified using quantitative reverse transcription PCR. RESULTS: The results showed no relationship between miRNA levels and biochemical variables on day 1. By day 7 of gentamicin treatment miR-15a-5p provided good discrimination between AKI and non-AKI (area under curve, 0.828). In patients taking vancomycin, miR-155-5p and miR-192-5p positively correlated with creatinine and NGAL values, and miR-192-5p and miR-423-5p positively correlated with procalcitonin and interleukin-6 in patients treated with a non-nephrotoxic antibiotic. In patients together we found positive correlation between miR-155-5p and miR-423-5p and all biochemical markers. CONCLUSION: The results suggest that these four miRNAs may serve as diagnostic or therapeutic tool in sepsis, renal injury and nephrotoxic treatment. TRIAL REGISTRATION: ClinicalTrials.gov , ID: NCT04991376 . Registered on 27 July 2021.

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$a BACKGROUND: Through regulation of signaling pathways, microRNAs (miRNAs) can be involved in sepsis and associated organ dysfunction. The aims of this study were to track the 7-day time course of blood miRNAs in patients with sepsis treated with vancomycin, gentamicin, or a non-nephrotoxic antibiotic and miRNA associations with neutrophil gelatinase-associated lipokalin (NGAL), creatinine, procalcitonin, interleukin-6, and acute kidney injury (AKI) stage. METHODS: Of 46 adult patients, 7 were on vancomycin, 20 on gentamicin, and 19 on another antibiotic. Blood samples were collected on days 1, 4, and 7 of treatment, and miRNAs were identified using quantitative reverse transcription PCR. RESULTS: The results showed no relationship between miRNA levels and biochemical variables on day 1. By day 7 of gentamicin treatment miR-15a-5p provided good discrimination between AKI and non-AKI (area under curve, 0.828). In patients taking vancomycin, miR-155-5p and miR-192-5p positively correlated with creatinine and NGAL values, and miR-192-5p and miR-423-5p positively correlated with procalcitonin and interleukin-6 in patients treated with a non-nephrotoxic antibiotic. In patients together we found positive correlation between miR-155-5p and miR-423-5p and all biochemical markers. CONCLUSION: The results suggest that these four miRNAs may serve as diagnostic or therapeutic tool in sepsis, renal injury and nephrotoxic treatment. TRIAL REGISTRATION: ClinicalTrials.gov , ID: NCT04991376 . Registered on 27 July 2021.
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$a Pribylova, Lenka $u Department of Applied Mathematics, Faculty of Electrical Engineering and Computer Science, VSB Technical University of Ostrava, 70800, Ostrava, Czech Republic
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$a Bris, Radim $u Department of Applied Mathematics, Faculty of Electrical Engineering and Computer Science, VSB Technical University of Ostrava, 70800, Ostrava, Czech Republic
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$a Kanova, Marcela $u Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic $u Department of Anesthesiology and Resuscitation, University Hospital Ostrava, 70852, Ostrava, Czech Republic
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$a Sigutova, Radka $u Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic $u Department of Epidemiology and Public Health, Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic $u Department of Laboratory Diagnostics Institute of Clinical Biochemistry and Clinical Pharmacology, University Hospital Ostrava, 70852, Ostrava, Czech Republic
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$a Kacirova, Ivana $u Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic $u Department of Laboratory Diagnostics Institute of Clinical Biochemistry and Clinical Pharmacology, University Hospital Ostrava, 70852, Ostrava, Czech Republic
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$a Bace, Eva $u Department of Immunodiagnostics, BioVendor R&D, Laboratory Medicine Corp, 621 00, Brno, Czech Republic
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$a Stejskal, David $u Faculty of Medicine, University of Ostrava, 70300, Ostrava, Czech Republic $u Department of Laboratory Diagnostics Institute of Clinical Biochemistry and Clinical Pharmacology, University Hospital Ostrava, 70852, Ostrava, Czech Republic
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