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Discovery of Long Non-Coding RNA MALAT1 Amplification in Precancerous Colorectal Lesions

A. Siskova, J. Kral, J. Drabova, K. Cervena, K. Tomasova, J. Jungwirth, T. Hucl, P. Kohout, S. Summerova, L. Vodickova, P. Vodicka, V. Vymetalkova

. 2022 ; 23 (14) : . [pub] 20220711

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22025245

Grantová podpora
AZV NV18-03-00199 Grant Agency of the Ministry of Health of the Czech Republic
GACR 22-05942S Grant Agency of the Czech Republic
Oncology and Haematology Cooperatio Program
CZ.02.2.69/0.0/0.0/19_073 project n. START/MED/052 Grant Schemes project at UK

A colorectal adenoma, an aberrantly growing tissue, arises from the intestinal epithelium and is considered as precursor of colorectal cancer (CRC). In this study, we investigated structural and numerical chromosomal aberrations in adenomas, hypothesizing that chromosomal instability (CIN) occurs early in adenomas. We applied array comparative genomic hybridization (aCGH) to fresh frozen colorectal adenomas and their adjacent mucosa from 16 patients who underwent colonoscopy examination. In our study, histologically similar colorectal adenomas showed wide variability in chromosomal instability. Based on the obtained results, we further stratified patients into four distinct groups. The first group showed the gain of MALAT1 and TALAM1, long non-coding RNAs (lncRNAs). The second group involved patients with numerous microdeletions. The third group consisted of patients with a disrupted karyotype. The fourth group of patients did not show any CIN in adenomas. Overall, we identified frequent losses in genes, such as TSC2, COL1A1, NOTCH1, MIR4673, and GNAS, and gene gain containing MALAT1 and TALAM1. Since long non-coding RNA MALAT1 is associated with cancer cell metastasis and migration, its gene amplification represents an important event for adenoma development.

Citace poskytuje Crossref.org

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