-
Je něco špatně v tomto záznamu ?
Isolation and characterization of thermostable and alkali-tolerant cellulase from litter endophytic fungus Bartalinia pondoensis
R. Yadav, M. Vasundhara, T. Rajamani, TS. Suryanarayanan, SM. Reddy
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- alkálie MeSH
- Ascomycota * metabolismus MeSH
- celulasa * chemie MeSH
- celulasy * MeSH
- endofyty metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- Publikační typ
- časopisecké články MeSH
Endophytic fungi in plant tissues produce a wide range of secondary metabolites and enzymes, which exhibit a variety of biological activities. In the present study, litter endophytic fungi were isolated from a fire-prone forest and screened for thermostable cellulases. Among nine endophytic fungi tested, two isolates, Bartalinia pondoensis and Phoma sp., showed the maximum cellulase activity. Bartalinia pondoensis was further selected for its cellulase production and characterization. Among the carbon and nitrogen sources tested, maximum cellulase production was observed with maltose and yeast extract, and the eucalyptus leaves and rice bran served as the best natural substrates. The cellulase activity increased with increasing temperature, with maximum activity recorded at 100 °C. The maximum CMCase activity was observed between pH 6.0 and 7.0 and retained 80% of its activity in the pH range of 8-10. Partially purified cellulase of B. pondoensis retained 50% of its activity after 2 h of incubation at 60 °C, 80 °C and 100 °C. These results suggest that litter endophytic fungus B. pondoensis is a potential source for the production of thermostable and alkali-tolerant cellulase.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22027720
- 003
- CZ-PrNML
- 005
- 20240416153157.0
- 007
- ta
- 008
- 221206s2022 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-022-00991-4 $2 doi
- 035 __
- $a (PubMed)35906455
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Yadav, Rajnish $u Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004, Punjab, India
- 245 10
- $a Isolation and characterization of thermostable and alkali-tolerant cellulase from litter endophytic fungus Bartalinia pondoensis / $c R. Yadav, M. Vasundhara, T. Rajamani, TS. Suryanarayanan, SM. Reddy
- 520 9_
- $a Endophytic fungi in plant tissues produce a wide range of secondary metabolites and enzymes, which exhibit a variety of biological activities. In the present study, litter endophytic fungi were isolated from a fire-prone forest and screened for thermostable cellulases. Among nine endophytic fungi tested, two isolates, Bartalinia pondoensis and Phoma sp., showed the maximum cellulase activity. Bartalinia pondoensis was further selected for its cellulase production and characterization. Among the carbon and nitrogen sources tested, maximum cellulase production was observed with maltose and yeast extract, and the eucalyptus leaves and rice bran served as the best natural substrates. The cellulase activity increased with increasing temperature, with maximum activity recorded at 100 °C. The maximum CMCase activity was observed between pH 6.0 and 7.0 and retained 80% of its activity in the pH range of 8-10. Partially purified cellulase of B. pondoensis retained 50% of its activity after 2 h of incubation at 60 °C, 80 °C and 100 °C. These results suggest that litter endophytic fungus B. pondoensis is a potential source for the production of thermostable and alkali-tolerant cellulase.
- 650 12
- $a celulasa $x chemie $7 D002480
- 650 _2
- $a alkálie $7 D000468
- 650 12
- $a Ascomycota $x metabolismus $7 D001203
- 650 12
- $a celulasy $7 D044602
- 650 _2
- $a endofyty $x metabolismus $7 D060026
- 650 _2
- $a koncentrace vodíkových iontů $7 D006863
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Vasundhara, Mondem $u Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004, Punjab, India
- 700 1_
- $a Rajamani, Thavamani $u Vivekananda Institute of Tropical Mycology (VINSTROM), Ramakrishna Mission Vidyapith, Chennai, 600004, Tamil Nadu, India
- 700 1_
- $a Suryanarayanan, Trichur S $u Vivekananda Institute of Tropical Mycology (VINSTROM), Ramakrishna Mission Vidyapith, Chennai, 600004, Tamil Nadu, India
- 700 1_
- $a Reddy, Sudhakara M $u Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, 147004, Punjab, India. msreddy@thapar.edu $1 https://orcid.org/0000000297434993
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 67, č. 6 (2022), s. 955-964
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/35906455 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20221206 $b ABA008
- 991 __
- $a 20240416153154 $b ABA008
- 999 __
- $a ok $b bmc $g 1867315 $s 1179033
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2022 $b 67 $c 6 $d 955-964 $e 20220729 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
- LZP __
- $a Pubmed-20221206
