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HITS-CLIP analysis of human ALKBH8 reveals interactions with fully processed substrate tRNAs and with specific noncoding RNAs
I. Cavallin, M. Bartosovic, T. Skalicky, P. Rengaraj, M. Demko, MC. Schmidt-Dengler, A. Drino, M. Helm, S. Vanacova
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 1995 do Před 6 měsíci
PubMed Central
od 1995 do Před 1 rokem
Europe PubMed Central
od 1995 do Před 1 rokem
Open Access Digital Library
od 1995-03-01
PubMed
36192131
DOI
10.1261/rna.079421.122
Knihovny.cz E-zdroje
- MeSH
- AlkB homolog 8, tRNA methyltransferasa genetika MeSH
- antikodon MeSH
- ChiP sekvenování * MeSH
- lidé MeSH
- nekódující RNA genetika MeSH
- RNA transferová * genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Transfer RNAs acquire a large plethora of chemical modifications. Among those, modifications of the anticodon loop play important roles in translational fidelity and tRNA stability. Four human wobble U-containing tRNAs obtain 5-methoxycarbonylmethyluridine (mcm5U34) or 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U34), which play a role in decoding. This mark involves a cascade of enzymatic activities. The last step is mediated by alkylation repair homolog 8 (ALKBH8). In this study, we performed a transcriptome-wide analysis of the repertoire of ALKBH8 RNA targets. Using a combination of HITS-CLIP and RIP-seq analyses, we uncover ALKBH8-bound RNAs. We show that ALKBH8 targets fully processed and CCA modified tRNAs. Our analyses uncovered the previously known set of wobble U-containing tRNAs. In addition, both our approaches revealed ALKBH8 binding to several other types of noncoding RNAs, in particular C/D box snoRNAs.
Central European Institute of Technology Masaryk University 625 00 Brno Czech Republic
Medical University of Vienna Center for Anatomy and Cell Biology 1090 Vienna Austria
Citace poskytuje Crossref.org
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