Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Sex differences in long-term effects of collagen-induced arthritis in middle-aged mice

BM. Schuh, K. Macáková, A. Feješ, T. Groß, P. Belvončíková, J. Janko, D. Juskanič, S. Hollý, V. Borbélyová, E. Šteňová, M. Pastorek, B. Vlková, P. Celec

. 2023 ; 14 (-) : 1195604. [pub] 20230628

Status neindexováno Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc23015845

Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory disorder with high prevalence among middle-aged women. Collagen-induced arthritis (CIA) is the most widely used animal model of RA, however, sex differences and long-term effects of CIA in mice are poorly described in the literature. Aim: Therefore, the present study aimed to analyze the long-term effects of CIA on the joints of middle-aged mice of both sexes and to describe potential sex differences. Materials and methods: CIA was induced in middle-aged DBA/1J mice by immunization with bovine type II collagen and complete Freund's adjuvant. Saline was administered to control mice. Arthritis score assessment, plethysmometry, and thermal imaging of the joints were performed weekly for 15 weeks. Locomotor activity, micro-computed tomography, joint histology and biochemical analyses were performed at the end of the experiment. Results: Our results indicate a similar prevalence of arthritis in both sexes of mice-67% (8/12) of females and 89% (8/9) males with an earlier onset in males (day 14 vs. day 35). After the arthritis scores peaked on day 56 for males and day 63 for females, they steadily declined until the end of the experiment on day 105. A similar dynamics was observed in paw volume and temperature analyzing different aspects of joint inflammation. Long-term consequences including higher proteinuria (by 116%), loss of bone density (by 33.5%) and joint damage in terms of synovial hyperplasia as well as bone and cartilage erosions were more severe in CIA males compared to CIA females. There were no significant differences in locomotor activity between CIA mice and CTRL mice of any sex. Conclusion: This is the first study to describe the long-term effects of the CIA model in terms of sex differences in DBA/1J mice. Our results indicate sex differences in the dynamics, but not in the extent of arthritis. An earlier onset of arthritis and more severe consequences on joints, bones and kidneys were found in males. The underlying immune pathomechanisms responsible for the limited duration of the arthritis symptoms and the opposite sex difference in comparison to RA patients require further investigation.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23015845
003      
CZ-PrNML
005      
20250717083529.0
007      
ta
008      
231010e20230628sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fphys.2023.1195604 $2 doi
035    __
$a (PubMed)37449011
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Schuh, Bernhard Maximilian $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
245    10
$a Sex differences in long-term effects of collagen-induced arthritis in middle-aged mice / $c BM. Schuh, K. Macáková, A. Feješ, T. Groß, P. Belvončíková, J. Janko, D. Juskanič, S. Hollý, V. Borbélyová, E. Šteňová, M. Pastorek, B. Vlková, P. Celec
520    9_
$a Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory disorder with high prevalence among middle-aged women. Collagen-induced arthritis (CIA) is the most widely used animal model of RA, however, sex differences and long-term effects of CIA in mice are poorly described in the literature. Aim: Therefore, the present study aimed to analyze the long-term effects of CIA on the joints of middle-aged mice of both sexes and to describe potential sex differences. Materials and methods: CIA was induced in middle-aged DBA/1J mice by immunization with bovine type II collagen and complete Freund's adjuvant. Saline was administered to control mice. Arthritis score assessment, plethysmometry, and thermal imaging of the joints were performed weekly for 15 weeks. Locomotor activity, micro-computed tomography, joint histology and biochemical analyses were performed at the end of the experiment. Results: Our results indicate a similar prevalence of arthritis in both sexes of mice-67% (8/12) of females and 89% (8/9) males with an earlier onset in males (day 14 vs. day 35). After the arthritis scores peaked on day 56 for males and day 63 for females, they steadily declined until the end of the experiment on day 105. A similar dynamics was observed in paw volume and temperature analyzing different aspects of joint inflammation. Long-term consequences including higher proteinuria (by 116%), loss of bone density (by 33.5%) and joint damage in terms of synovial hyperplasia as well as bone and cartilage erosions were more severe in CIA males compared to CIA females. There were no significant differences in locomotor activity between CIA mice and CTRL mice of any sex. Conclusion: This is the first study to describe the long-term effects of the CIA model in terms of sex differences in DBA/1J mice. Our results indicate sex differences in the dynamics, but not in the extent of arthritis. An earlier onset of arthritis and more severe consequences on joints, bones and kidneys were found in males. The underlying immune pathomechanisms responsible for the limited duration of the arthritis symptoms and the opposite sex difference in comparison to RA patients require further investigation.
590    __
$a NEINDEXOVÁNO
655    _2
$a časopisecké články $7 D016428
700    1_
$a Macáková, Kristína $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
700    1_
$a Feješ, Andrej $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
700    1_
$a Groß, Tim $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
700    1_
$a Belvončíková, Paulína $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
700    1_
$a Janko, Jakub, $d 1990- $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia $7 xx0334142
700    1_
$a Juskanič, Dominik $u Jessenius-Diagnostic Center, Nitra, Slovakia $u Faculty of Medicine, Comenius University, Bratislava, Slovakia
700    1_
$a Hollý, Samuel $u Jessenius-Diagnostic Center, Nitra, Slovakia $u First Faculty of Medicine, Institute of Biophysics and Informatics, Charles University, Prague, Czechia
700    1_
$a Borbélyová, Veronika $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
700    1_
$a Šteňová, Emőke $u 1st Department of Internal Medicine, Faculty of Medicine, University Hospital, Comenius University, Bratislava, Slovakia
700    1_
$a Pastorek, Michal $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
700    1_
$a Vlková, Barbora $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia
700    1_
$a Celec, Peter $u Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia $u Faculty of Medicine, Institute of Pathophysiology, Comenius University, Bratislava, Slovakia
773    0_
$w MED00174601 $t Frontiers in physiology $x 1664-042X $g Roč. 14 (20230628), s. 1195604
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37449011 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20231010 $b ABA008
991    __
$a 20250717083511 $b ABA008
999    __
$a ok $b bmc $g 1997289 $s 1202207
BAS    __
$a 3
BAS    __
$a PreBMC-PubMed-not-MEDLINE
BMC    __
$a 2023 $b 14 $c - $d 1195604 $e 20230628 $i 1664-042X $m Frontiers in physiology $n Front. physiol. $x MED00174601
LZP    __
$a Pubmed-20231010

Najít záznam

Citační ukazatele

Možnosti archivace