• Je něco špatně v tomto záznamu ?

Studying molecular interactions via capillary electrophoresis and microscale thermophoresis: A review

M. Asmari, L. Michalcová, AE. Ibrahim, Z. Glatz, H. Wätzig, S. El Deeb

. 2023 ; 44 (13-14) : 1114-1142. [pub] 20230424

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, přehledy, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc23017013

The process of choosing the most proper technique for studying the molecular interactions is based on critical factors such as instrumentation complexity, automation, experimental procedures, analysis time, consumables, and cost-value. This review has tracked the use of affinity capillary electrophoresis (ACE) and microscale thermophoresis (MST) techniques in the evaluation of molecular binding among different molecules during the 5 years 2016-2021. ACE has proved to be an attractive technique for biomolecular characterization with high resolution efficiency where small variations in several controlling factors can much improve such efficiency compared to other analytical techniques. Meanwhile, MST has proved its higher sensitivity for smaller amounts of complex non-purified biosamples without affecting its robustness while providing high through output. However, the main motivation to review both techniques in the proposed review was their capability to carry out all experiments without the need for immobilizing one interacting partner, besides a great flexibility in the use of buffering systems. The proposed review demonstrates the importance of both techniques in different areas of life sciences. Moreover, the recent advances in exploiting ACE and MST in other research interests have been discussed.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23017013
003      
CZ-PrNML
005      
20231026105336.0
007      
ta
008      
231013s2023 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/elps.202200275 $2 doi
035    __
$a (PubMed)37043774
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Asmari, Mufarreh $u College of Pharmacy, King Khalid University, Abha, Saudi Arabia
245    10
$a Studying molecular interactions via capillary electrophoresis and microscale thermophoresis: A review / $c M. Asmari, L. Michalcová, AE. Ibrahim, Z. Glatz, H. Wätzig, S. El Deeb
520    9_
$a The process of choosing the most proper technique for studying the molecular interactions is based on critical factors such as instrumentation complexity, automation, experimental procedures, analysis time, consumables, and cost-value. This review has tracked the use of affinity capillary electrophoresis (ACE) and microscale thermophoresis (MST) techniques in the evaluation of molecular binding among different molecules during the 5 years 2016-2021. ACE has proved to be an attractive technique for biomolecular characterization with high resolution efficiency where small variations in several controlling factors can much improve such efficiency compared to other analytical techniques. Meanwhile, MST has proved its higher sensitivity for smaller amounts of complex non-purified biosamples without affecting its robustness while providing high through output. However, the main motivation to review both techniques in the proposed review was their capability to carry out all experiments without the need for immobilizing one interacting partner, besides a great flexibility in the use of buffering systems. The proposed review demonstrates the importance of both techniques in different areas of life sciences. Moreover, the recent advances in exploiting ACE and MST in other research interests have been discussed.
650    12
$a elektroforéza kapilární $x metody $7 D019075
650    _2
$a vazba proteinů $7 D011485
655    _2
$a časopisecké články $7 D016428
655    _2
$a přehledy $7 D016454
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Michalcová, Lenka $u Department of Biochemistry, Masaryk University, Brno, Czech Republic $1 https://orcid.org/0000000248801304
700    1_
$a Ibrahim, Adel Ehab $u Pharmaceutical Analytical chemistry department, Faculty of Pharmacy, Port-Said University, Port Said, Egypt $u Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Sultanate of Oman $1 https://orcid.org/0000000174211220
700    1_
$a Glatz, Zdeněk $u Department of Biochemistry, Masaryk University, Brno, Czech Republic $1 https://orcid.org/0000000334266117
700    1_
$a Wätzig, Hermann $u Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany $1 https://orcid.org/0000000274560612
700    1_
$a El Deeb, Sami $u Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Sultanate of Oman $u Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany
773    0_
$w MED00001508 $t Electrophoresis $x 1522-2683 $g Roč. 44, č. 13-14 (2023), s. 1114-1142
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37043774 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20231013 $b ABA008
991    __
$a 20231026105331 $b ABA008
999    __
$a ok $b bmc $g 2000501 $s 1203375
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 44 $c 13-14 $d 1114-1142 $e 20230424 $i 1522-2683 $m Electrophoresis $n Electrophoresis $x MED00001508
LZP    __
$a Pubmed-20231013

Najít záznam

Citační ukazatele

Možnosti archivace