• Something wrong with this record ?

Animal experimental models of ischemic limbs - A systematic review

V. Lovasova, R. Bem, J. Chlupac, M. Dubsky, J. Husakova, A. Nemcova, J. Fronek

. 2023 ; 153 (-) : 107237. [pub] 20231005

Language English Country United States

Document type Systematic Review, Journal Article, Review

Persistent link   https://www.medvik.cz/link/bmc24000441

BACKGROUND: The objective of this systematic review is to summarize the available animal models of ischemic limbs, and to provide an overview of the advantages and disadvantages of each animal model and individual method of limb ischemia creation. METHODS: A review of literature was conducted using the PubMed and Web of Science pages. Various types of experimental animals and surgical approaches used in creating ischemic limbs were evaluated. Other outcomes of interest were the specific characteristics of the individual experimental animals, and duration of tissue ischemia. RESULTS: The most commonly used experimental animals were mice, followed by rabbits, rats, pigs, miniature pigs, and sheep. Single or double arterial ligation and excision of the entire femoral artery was the most often used method of ischemic limb creation. Other methods comprised single or double arterial electrocoagulation, use of ameroid constrictors, photochemically induced thrombosis, and different types of endovascular methods. The shortest duration of tissue ischemia was 7 days, the longest 90 days. CONCLUSIONS: This review shows that mice are among the most commonly used animals in limb ischemia research. Simple ligation and excision of the femoral artery is the most common method of creating an ischemic limb; nevertheless, it can result in acute rather than chronic ischemia. A two-stage sequential approach and methods using ameroid constrictors or endovascular blinded stent grafts are more suitable for creating a gradual arterial occlusion typically seen in humans. Selecting the right mouse strain or animal with artificially produced diabetes or hyperlipidaemia is crucial in chronic ischemic limb research. Moreover, the observation period following the onset of ischemia should last at least 14 days, preferably 4 weeks.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24000441
003      
CZ-PrNML
005      
20250507092531.0
007      
ta
008      
240109e20231005xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.vph.2023.107237 $2 doi
035    __
$a (PubMed)37802406
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Lovasova, Veronika $u Transplant Surgery Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; Second Faculty of Medicine, Charles University, Prague, Czech Republic. Electronic address: veronika.lovasova@ikem.cz
245    10
$a Animal experimental models of ischemic limbs - A systematic review / $c V. Lovasova, R. Bem, J. Chlupac, M. Dubsky, J. Husakova, A. Nemcova, J. Fronek
520    9_
$a BACKGROUND: The objective of this systematic review is to summarize the available animal models of ischemic limbs, and to provide an overview of the advantages and disadvantages of each animal model and individual method of limb ischemia creation. METHODS: A review of literature was conducted using the PubMed and Web of Science pages. Various types of experimental animals and surgical approaches used in creating ischemic limbs were evaluated. Other outcomes of interest were the specific characteristics of the individual experimental animals, and duration of tissue ischemia. RESULTS: The most commonly used experimental animals were mice, followed by rabbits, rats, pigs, miniature pigs, and sheep. Single or double arterial ligation and excision of the entire femoral artery was the most often used method of ischemic limb creation. Other methods comprised single or double arterial electrocoagulation, use of ameroid constrictors, photochemically induced thrombosis, and different types of endovascular methods. The shortest duration of tissue ischemia was 7 days, the longest 90 days. CONCLUSIONS: This review shows that mice are among the most commonly used animals in limb ischemia research. Simple ligation and excision of the femoral artery is the most common method of creating an ischemic limb; nevertheless, it can result in acute rather than chronic ischemia. A two-stage sequential approach and methods using ameroid constrictors or endovascular blinded stent grafts are more suitable for creating a gradual arterial occlusion typically seen in humans. Selecting the right mouse strain or animal with artificially produced diabetes or hyperlipidaemia is crucial in chronic ischemic limb research. Moreover, the observation period following the onset of ischemia should last at least 14 days, preferably 4 weeks.
650    _2
$a lidé $7 D006801
650    _2
$a zvířata $7 D000818
650    _2
$a myši $7 D051379
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a králíci $7 D011817
650    _2
$a prasata $7 D013552
650    _2
$a ovce $7 D012756
650    _2
$a modely u zvířat $7 D023421
650    12
$a ischemie $7 D007511
650    12
$a arteria femoralis $x chirurgie $7 D005263
650    _2
$a stenty $7 D015607
650    _2
$a teoretické modely $7 D008962
650    _2
$a modely nemocí na zvířatech $7 D004195
655    _2
$a systematický přehled $7 D000078182
655    _2
$a časopisecké články $7 D016428
655    _2
$a přehledy $7 D016454
700    1_
$a Bem, Robert $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Chlupac, Jaroslav $u Transplant Surgery Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; Department of Anatomy, Second Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Dubsky, Michal $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Husakova, Jitka $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Němcová, Andrea $u Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic $7 xx0231185
700    1_
$a Fronek, Jiri $u Transplant Surgery Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; First Faculty of Medicine, Charles University, Prague, Czech Republic; Department of Anatomy, Second Faculty of Medicine, Charles University, Prague, Czech Republic
773    0_
$w MED00008593 $t Vascular pharmacology $x 1879-3649 $g Roč. 153 (20231005), s. 107237
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37802406 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240109 $b ABA008
991    __
$a 20250507092529 $b ABA008
999    __
$a ok $b bmc $g 2049233 $s 1210135
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 153 $c - $d 107237 $e 20231005 $i 1879-3649 $m Vascular pharmacology $n Vascul Pharmacol $x MED00008593
LZP    __
$a Pubmed-20240109

Find record

Citation metrics

Archiving options