Streptococcus thermophilus, the only Streptococcus species considered "Generally Recognized Safe", has been used widely in the food industry. This bacterium is one of the most valuable industrial lactic acid bacterial species. Due to the importance of this bacterium in industrial applications, it should be stored for a long time without losing its metabolic properties. The present study aimed to investigate the cryoprotectant effect of three compatible solutes (ectoine, trehalose, and sucrose) on bacterial cells stored at different temperatures (frozen at -80 °C or freeze-dried and subsequently stored at +4, -20, and -80 °C) for three months. The bacterial cells were tested for cell viability, bile salt tolerance, and lactic acid production before and after processing. The highest cell viability, bile salt tolerance, and lactic acid production were obtained with ectoine and under frozen (storage at -80 °C) conditions. In freeze-dried and subsequently stored at various temperatures, the best preservation was obtained at -80 °C, followed by -20 °C and +4 °C. Moreover, when ectoine's preservation potential was compared to other cryoprotectants, ectoine showed the highest preservation, followed by trehalose and sucrose. Although ectoine has a variety of qualities that have been proven, in the current work, we have shown for the first time that ectoine has cryoprotectant potential in yogurt starter cultures (S. thermophilus).

Art and Science Faculty Department of Molecular Biology and Genetics Agri Ibrahim Cecen University Agri 4100 Türkiye

Central Research and Application Laboratory Agri Ibrahim Cecen University Agri 4100 Türkiye

Department of Medicinal and Aromatic Plants Giresun University Giresun Türkiye

Department of Molecular Biology and Genetics Mus Alparslan University Mus 49250 Türkiye

Engineering Faculty Basic Sciences Department Samsun University Samsun 55420 Türkiye

000      

00000naa a2200000 a 4500

001      

bmc24003884

003      

CZ-PrNML

005      

20240305161720.0

007      

ta

008      

240305s2024 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1007/s12223-023-01098-0 $2 doi

035    __

$a (PubMed)37917277

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xr

100    1_

$a Orhan, Furkan $u Art and Science Faculty, Department of Molecular Biology and Genetics, Agri Ibrahim Cecen University, Agri, 4100, Türkiye. furkan_orhan@hotmail.com $u Central Research and Application Laboratory, Agri Ibrahim Cecen University, Agri, 4100, Türkiye. furkan_orhan@hotmail.com $1 https://orcid.org/0000000169066604

245    10

$a Usage of ectoine as a cryoprotectant for cryopreservation of lactic acid bacteria / $c F. Orhan, A. Demirci, D. Efe, R. Aydın, S. Bozarı

520    9_

$a Streptococcus thermophilus, the only Streptococcus species considered "Generally Recognized Safe", has been used widely in the food industry. This bacterium is one of the most valuable industrial lactic acid bacterial species. Due to the importance of this bacterium in industrial applications, it should be stored for a long time without losing its metabolic properties. The present study aimed to investigate the cryoprotectant effect of three compatible solutes (ectoine, trehalose, and sucrose) on bacterial cells stored at different temperatures (frozen at -80 °C or freeze-dried and subsequently stored at +4, -20, and -80 °C) for three months. The bacterial cells were tested for cell viability, bile salt tolerance, and lactic acid production before and after processing. The highest cell viability, bile salt tolerance, and lactic acid production were obtained with ectoine and under frozen (storage at -80 °C) conditions. In freeze-dried and subsequently stored at various temperatures, the best preservation was obtained at -80 °C, followed by -20 °C and +4 °C. Moreover, when ectoine's preservation potential was compared to other cryoprotectants, ectoine showed the highest preservation, followed by trehalose and sucrose. Although ectoine has a variety of qualities that have been proven, in the current work, we have shown for the first time that ectoine has cryoprotectant potential in yogurt starter cultures (S. thermophilus).

650    12

$a Lactobacillales $7 D056546

650    _2

$a trehalosa $7 D014199

650    _2

$a kryoprezervace $7 D015925

650    _2

$a kryoprotektivní látky $x farmakologie $7 D003451

650    _2

$a kyselina mléčná $7 D019344

650    _2

$a sacharosa $7 D013395

650    12

$a aminokyseliny diaminové $7 D000599

655    _2

$a časopisecké články $7 D016428

700    1_

$a Demirci, Abdullah $u Central Research and Application Laboratory, Agri Ibrahim Cecen University, Agri, 4100, Türkiye $1 https://orcid.org/0000000204225632

700    1_

$a Efe, Derya $u Department of Medicinal and Aromatic Plants, Giresun University, Giresun, Türkiye $1 https://orcid.org/0000000335541790

700    1_

$a Aydın, Rukiye $u Engineering Faculty, Basic Sciences Department, Samsun University, Samsun, 55420, Türkiye $1 https://orcid.org/0000000305761354

700    1_

$a Bozarı, Sedat $u Department of Molecular Biology and Genetics, Mus Alparslan University, Mus, 49250, Türkiye $1 https://orcid.org/0000000152652236

773    0_

$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 69, č. 1 (2024), s. 133-144

856    41

$u https://pubmed.ncbi.nlm.nih.gov/37917277 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y - $z 0

990    __

$a 20240305 $b ABA008

991    __

$a 20240305161716 $b ABA008

999    __

$a ok $b bmc $g 2059333 $s 1213636

BAS    __

$a 3

BAS    __

$a PreBMC-MEDLINE

BMC    __

$a 2024 $b 69 $c 1 $d 133-144 $e 20231102 $i 1874-9356 $m Folia microbiologica $n Folia Microbiol (Praha) $x MED00011005

LZP    __

$a Pubmed-20240305