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Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay
C. Aira, G. González-García, J. Martínez-Cano, N. de la Roja, M. Giammarioli, F. Feliziani, Ž. Šteingolde, J. Buitkuviene, P. Václavek, D. Glišić, C. Gallardo, P. Sastre, M. García-Durán, P. Rueda, A. Fresco-Taboada
Status not-indexed Language English Country Switzerland
Document type Journal Article
Grant support
862874
European Commission
NLK
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- Publication type
- Journal Article MeSH
African swine fever (ASF) is a contagious disease of wild boar and domestic pigs notifiable to the World Organisation for Animal Health due to its high socio-economic impact. ASF is caused by the complex ASF virus (ASFV), and it can present different clinical manifestations that can be confused with other diseases; for this reason, laboratory testing is necessary for the proper diagnosis of clinically suspected animals. Despite the efforts put into it over decades, no treatment or safe vaccine is globally available, and disease control is based on early diagnosis and the implementation of strict biosecurity measures. In this context, rapid tests have the potential to accelerate and facilitate the identification of infected animals by giving fast on-site results. In this work, we improved the available point-of-care assays for the diagnosis of the disease by the development of a more specific antigen test and a more sensitive antibody test. This antibody detection test allowed for the earlier detection of infected animals than two commercial indirect ELISAs (statistically significant). Moreover, we developed a combined dual rapid test, unifying, in the same cassette, an antigen detection strip and an antibody detection strip. In this study, we confirmed that this combo approach is a useful tool for implementing rapid tests in the field since it increases the percentage of positive samples detected, even when PCR turns negative, while maintaining a good specificity.
Gold Standard Diagnostics Madrid Calle de los Hermanos García Noblejas 39 28037 Madrid Spain
Institute of Food Safety Animal Health and Environment BIOR Lejupes Street 3 LV 1076 Riga Latvia
National Food and Veterinary Risk Assessment Institute J Kairiūkščio g 10 LT 08409 Vilnius Lithuania
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