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Hand2 Immunohistochemistry in the Diagnosis of Paragangliomas and Other Neuroendocrine Neoplasms
J. Soukup, M. Manethova, V. Stejskal, M. Novakova, J. Duskova, H. Hornychova, M. Hacova, I. Staniczkova-Zambo, T. Zelinka, M. Kosak, T. Cesak, D. Netuka, A. Ryska, F. Gabalec
Language English Country United States
Document type Journal Article
Grant support
LM2023033
BBMRI-CZ
Cooperatio DIAG
Univerzita Karlova v Praze
Cooperatio METD
Univerzita Karlova v Praze
EF16_013/0001674
European Regional Development Fund-Project BBMRI-CZ
conceptual development of research organization 00064165
Ministerstvo Zdravotnictví Ceské Republiky
NU23-01-00323
Ministerstvo Zdravotnictví Ceské Republiky
22-07091S
Grantová Agentura České Republiky
MO 1012
Ministerstvo Obrany České Republiky
NLK
ProQuest Central
from 1997-04-01 to 1 year ago
Medline Complete (EBSCOhost)
from 1997-03-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-04-01 to 1 year ago
- MeSH
- Immunohistochemistry MeSH
- Humans MeSH
- Carcinoma, Neuroendocrine * pathology MeSH
- Neuroendocrine Tumors * pathology MeSH
- Paraganglioma * diagnosis pathology MeSH
- Transcription Factors metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
Hand2 is a core transcription factor responsible for chromaffin cell differentiation. However, its potential utility in surgical pathology has not been studied. Thus, we aimed to investigate its expression in paragangliomas, other neuroendocrine neoplasms (NENs), and additional non-neuroendocrine tumors. We calibrated Hand2 immunohistochemistry on adrenal medulla cells and analyzed H-scores in 46 paragangliomas (PGs), 9 metastatic PGs, 21 cauda equina neuroendocrine tumors (CENETs), 48 neuroendocrine carcinomas (NECs), 8 olfactory neuroblastomas (ONBs), 110 well-differentiated NETs (WDNETs), 10 adrenal cortical carcinomas, 29 adrenal cortical adenomas, 8 melanomas, 41 different carcinomas, and 10 gastrointestinal stromal tumors (GISTs). Both tissue microarrays (TMAs) and whole sections (WSs) were studied. In 171 NENs, previously published data on Phox2B and GATA3 were correlated with Hand2. Hand2 was positive in 98.1% (54/55) PGs, but only rarely in WDNETs (9.6%, 10/104), CENETs (9.5%, 2/21), NECs (4.2%, 2/48), or ONBs (12.5%, 1/8). Any Hand2 positivity was 98.1% sensitive and 91.7% specific for the diagnosis of PG. The Hand2 H-score was significantly higher in primary PGs compared to Hand2-positive WDNETs (median 166.3 vs. 7.5; p < 0.0001). Metastatic PGs were positive in 88.9% (8/9). No Hand2 positivity was observed in any adrenal cortical neoplasm or other non-neuroendocrine tumors, with exception of 8/10 GISTs. Parasympathetic PGs showed a higher Hand2 H-score compared to sympathetic PGs (median H-scores 280 vs. 104, p < 0.0001). Hand2 positivity in NENs serves as a reliable marker of primary and metastatic PG, since other NENs only rarely exhibit limited Hand2 positivity.
References provided by Crossref.org
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