• Something wrong with this record ?

Liquid chromatography-tandem mass spectrometry methods for quantification of total and free antibiotic concentrations in serum and exudate from patients with post-sternotomy deep sternal wound infection receiving negative pressure wound therapy

H. Brozmanová, P. Šištík, J. Ďuricová, I. Kacířová, K. Kaňková, M. Kolek

. 2024 ; 554 (-) : 117704. [pub] 20240106

Language English Country Netherlands

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24007304

BACKGROUND: Systemically administered antibiotics are thought to penetrate the wounds more effectively during negative pressure wound therapy (NPWT).To test this hypothesis total and free antibiotic concentrations were quantified in serum and wound exudate. METHODS: UHPLC-MS/MS methods were developed and validated for the determination of ceftazidime, cefepime, cefotaxime, cefuroxime, cefazolin, meropenem, oxacillin, piperacillin with tazobactam, clindamycin, ciprofloxacin, sulfamethoxazole/trimethoprim (cotrimoxazole), gentamicin, vancomycin, and linezolid. The unbound antibiotic fraction was obtained by ultrafiltration using a Millipore Microcon-30kda Centrifugal Filter Unit. Analysis was performed on a 1.7-μm Acquity UPLC BEH C18 2.1 × 100-mm column with a gradient elution. RESULTS: The validation was performed for serum, exudates and free fractions. For all matrices, requirements were met regarding linearity, precision, accuracy, limit of quantitation, and matrix effect. The coefficient of variation was in the range of 1.2-13.6%.and the recovery 87.6-115.6%, respectively. Among the 29 applications of antibiotics thus far, including vancomycin, clindamycin, ciprofloxacin, oxacillin, cefepime, cefotaxime, cotrimoxazole, and gentamicin, total and free antibiotic concentrations in serum and exudate were correlated. CONCLUSION: This method can accurately quantify the total and free concentrations of 16 antibiotics. Comparison of concentration ratios between serum and exudates allows for monitoring individual antibiotics' penetration capacity in patients receiving NPWT.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24007304
003      
CZ-PrNML
005      
20240423155850.0
007      
ta
008      
240412e20240106ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.cca.2023.117704 $2 doi
035    __
$a (PubMed)38185284
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Brozmanová, Hana $u Department of Clinical Pharmacology, Institute of Laboratory Medicine, University Hospital Ostrava, 17. listopadu 1790, 708 52 Ostrava, Czech Republic; Department of Clinical Pharmacology, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00 Ostrava, Czech Republic
245    10
$a Liquid chromatography-tandem mass spectrometry methods for quantification of total and free antibiotic concentrations in serum and exudate from patients with post-sternotomy deep sternal wound infection receiving negative pressure wound therapy / $c H. Brozmanová, P. Šištík, J. Ďuricová, I. Kacířová, K. Kaňková, M. Kolek
520    9_
$a BACKGROUND: Systemically administered antibiotics are thought to penetrate the wounds more effectively during negative pressure wound therapy (NPWT).To test this hypothesis total and free antibiotic concentrations were quantified in serum and wound exudate. METHODS: UHPLC-MS/MS methods were developed and validated for the determination of ceftazidime, cefepime, cefotaxime, cefuroxime, cefazolin, meropenem, oxacillin, piperacillin with tazobactam, clindamycin, ciprofloxacin, sulfamethoxazole/trimethoprim (cotrimoxazole), gentamicin, vancomycin, and linezolid. The unbound antibiotic fraction was obtained by ultrafiltration using a Millipore Microcon-30kda Centrifugal Filter Unit. Analysis was performed on a 1.7-μm Acquity UPLC BEH C18 2.1 × 100-mm column with a gradient elution. RESULTS: The validation was performed for serum, exudates and free fractions. For all matrices, requirements were met regarding linearity, precision, accuracy, limit of quantitation, and matrix effect. The coefficient of variation was in the range of 1.2-13.6%.and the recovery 87.6-115.6%, respectively. Among the 29 applications of antibiotics thus far, including vancomycin, clindamycin, ciprofloxacin, oxacillin, cefepime, cefotaxime, cotrimoxazole, and gentamicin, total and free antibiotic concentrations in serum and exudate were correlated. CONCLUSION: This method can accurately quantify the total and free concentrations of 16 antibiotics. Comparison of concentration ratios between serum and exudates allows for monitoring individual antibiotics' penetration capacity in patients receiving NPWT.
650    _2
$a lidé $7 D006801
650    _2
$a antibakteriální látky $7 D000900
650    _2
$a tandemová hmotnostní spektrometrie $x metody $7 D053719
650    _2
$a cefepim $7 D000077723
650    _2
$a vankomycin $7 D014640
650    _2
$a kombinace léků trimethoprim a sulfamethoxazol $7 D015662
650    _2
$a klindamycin $7 D002981
650    _2
$a sternotomie $7 D056346
650    12
$a terapie ran pomocí řízeného podtlaku $7 D054843
650    _2
$a chromatografie kapalinová $x metody $7 D002853
650    _2
$a ciprofloxacin $7 D002939
650    _2
$a cefotaxim $7 D002439
650    _2
$a oxacilin $7 D010068
650    12
$a infekce v ráně $7 D014946
650    _2
$a gentamiciny $7 D005839
650    _2
$a exsudáty a transsudáty $7 D005122
650    _2
$a vysokoúčinná kapalinová chromatografie $x metody $7 D002851
655    _2
$a časopisecké články $7 D016428
700    1_
$a Šištík, Pavel $u Department of Clinical Pharmacology, Institute of Laboratory Medicine, University Hospital Ostrava, 17. listopadu 1790, 708 52 Ostrava, Czech Republic; Department of Clinical Pharmacology, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00 Ostrava, Czech Republic. Electronic address: pavel.sistik@fno.cz
700    1_
$a Ďuricová, Jana $u Department of Clinical Pharmacology, Institute of Laboratory Medicine, University Hospital Ostrava, 17. listopadu 1790, 708 52 Ostrava, Czech Republic; Department of Clinical Pharmacology, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00 Ostrava, Czech Republic
700    1_
$a Kacířová, Ivana $u Department of Clinical Pharmacology, Institute of Laboratory Medicine, University Hospital Ostrava, 17. listopadu 1790, 708 52 Ostrava, Czech Republic; Department of Clinical Pharmacology, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00 Ostrava, Czech Republic
700    1_
$a Kaňková, Klára $u Department of Cardiac Surgery, University Hospital Ostrava, 17. listopadu 1790, 708 52 Ostrava, Czech Republic
700    1_
$a Kolek, Martin $u Department of Cardiac Surgery, University Hospital Ostrava, 17. listopadu 1790, 708 52 Ostrava, Czech Republic; Department of Clinic Subjects, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00 Ostrava, Czech Republic
773    0_
$w MED00009464 $t Clinica chimica acta $x 1873-3492 $g Roč. 554 (20240106), s. 117704
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38185284 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240412 $b ABA008
991    __
$a 20240423155846 $b ABA008
999    __
$a ok $b bmc $g 2081345 $s 1217071
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 554 $c - $d 117704 $e 20240106 $i 1873-3492 $m Clinica chimica acta $n Clin Chim Acta $x MED00009464
LZP    __
$a Pubmed-20240412

Find record

Citation metrics

Archiving options