-
Je něco špatně v tomto záznamu ?
Fibroblast-Epithelium Co-culture Methods Using Epithelial Organoids and Cell Line-Derived Spheroids
J. Sumbal, Z. Sumbalova Koledova
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- buněčné linie MeSH
- epitel metabolismus MeSH
- epitelové buňky * MeSH
- fibroblasty metabolismus MeSH
- karcinogeneze patologie MeSH
- kokultivační techniky MeSH
- lidé MeSH
- mléčné žlázy zvířat * MeSH
- organoidy MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Fibroblasts are an integral cell type of mammary gland stroma, which plays crucial roles in development, homeostasis, and tumorigenesis of mammary epithelium. Fibroblasts produce and remodel extracellular matrix proteins and secrete a plethora of paracrine signals, which instruct both epithelial and other stromal cells of the mammary gland through mechanisms, which have not been fully understood. To enable deciphering of the intricate fibroblast-epithelial interactions, we developed several 3D co-culture methods. In this chapter, we describe methods for establishment of various types of embedded 3D co-cultures of mammary fibroblasts with mammary epithelial organoids, mammary tumor organoids, or breast cancer spheroids to investigate the role of fibroblasts in mammary epithelial development, morphogenesis, and tumorigenesis. The co-culture types include dispersed, aggregated, and transwell cultures.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24007684
- 003
- CZ-PrNML
- 005
- 20240423160202.0
- 007
- ta
- 008
- 240412s2024 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-0716-3674-9_8 $2 doi
- 035 __
- $a (PubMed)38393591
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Sumbal, Jakub $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic $u Laboratory of Genetics and Developmental Biology, Institut Curie, INSERM U934, CNRS UMR3215, Paris, France $u Sorbonne Université, Collège Doctoral, Paris, France $1 https://orcid.org/0000000337004518
- 245 10
- $a Fibroblast-Epithelium Co-culture Methods Using Epithelial Organoids and Cell Line-Derived Spheroids / $c J. Sumbal, Z. Sumbalova Koledova
- 520 9_
- $a Fibroblasts are an integral cell type of mammary gland stroma, which plays crucial roles in development, homeostasis, and tumorigenesis of mammary epithelium. Fibroblasts produce and remodel extracellular matrix proteins and secrete a plethora of paracrine signals, which instruct both epithelial and other stromal cells of the mammary gland through mechanisms, which have not been fully understood. To enable deciphering of the intricate fibroblast-epithelial interactions, we developed several 3D co-culture methods. In this chapter, we describe methods for establishment of various types of embedded 3D co-cultures of mammary fibroblasts with mammary epithelial organoids, mammary tumor organoids, or breast cancer spheroids to investigate the role of fibroblasts in mammary epithelial development, morphogenesis, and tumorigenesis. The co-culture types include dispersed, aggregated, and transwell cultures.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a kokultivační techniky $7 D018920
- 650 12
- $a epitelové buňky $7 D004847
- 650 12
- $a mléčné žlázy zvířat $7 D008321
- 650 _2
- $a epitel $x metabolismus $7 D004848
- 650 _2
- $a buněčné linie $7 D002460
- 650 _2
- $a fibroblasty $x metabolismus $7 D005347
- 650 _2
- $a organoidy $7 D009940
- 650 _2
- $a karcinogeneze $x patologie $7 D063646
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Sumbalova Koledova, Zuzana $u Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. zuzana.sumbalova-koledova@img.cas.cz $u Laboratory of Tissue Morphogenesis and Cancer, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic. zuzana.sumbalova-koledova@img.cas.cz $1 https://orcid.org/0000000293331399 $7 jx20110603007
- 773 0_
- $w MED00180389 $t Methods in molecular biology $x 1940-6029 $g Roč. 2764 (2024), s. 107-129
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/38393591 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20240412 $b ABA008
- 991 __
- $a 20240423160158 $b ABA008
- 999 __
- $a ok $b bmc $g 2081586 $s 1217451
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2024 $b 2764 $c - $d 107-129 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20240412
