Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Toxicity of Large and Small Surface-Engineered Upconverting Nanoparticles for In Vitro and In Vivo Bioapplications

L. Machová Urdzíková, D. Mareková, T. Vasylyshyn, P. Matouš, V. Patsula, V. Oleksa, O. Shapoval, M. Vosmanská, D. Liebl, A. Benda, V. Herynek, D. Horák, P. Jendelová

. 2024 ; 25 (10) : . [pub] 20240513

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24013947

Grantová podpora
OPJAK CZ.02.01.01/00/22_008/0004562 Ministry of sports and education
No. 21-04420S Czech Science Foundation

In this study, spherical or hexagonal NaYF4:Yb,Er nanoparticles (UCNPs) with sizes of 25 nm (S-UCNPs) and 120 nm (L-UCNPs) were synthesized by high-temperature coprecipitation and subsequently modified with three kinds of polymers. These included poly(ethylene glycol) (PEG) and poly(N,N-dimethylacrylamide-co-2-aminoethylacrylamide) [P(DMA-AEA)] terminated with an alendronate anchoring group, and poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The internalization of nanoparticles by rat mesenchymal stem cells (rMSCs) and C6 cancer cells (rat glial tumor cell line) was visualized by electron microscopy and the cytotoxicity of the UCNPs and their leaches was measured by the real-time proliferation assay. The comet assay was used to determine the oxidative damage of the UCNPs. An in vivo study on mice determined the elimination route and potential accumulation of UCNPs in the body. The results showed that the L- and S-UCNPs were internalized into cells in the lumen of endosomes. The proliferation assay revealed that the L-UCNPs were less toxic than S-UCNPs. The viability of rMSCs incubated with particles decreased in the order S-UCNP@Ale-(PDMA-AEA) > S-UCNP@Ale-PEG > S-UCNPs > S-UCNP@PMVEMA. Similar results were obtained in C6 cells. The oxidative damage measured by the comet assay showed that neat L-UCNPs caused more oxidative damage to rMSCs than all coated UCNPs while no difference was observed in C6 cells. An in vivo study indicated that L-UCNPs were eliminated from the body via the hepatobiliary route; L-UCNP@Ale-PEG particles were almost eliminated from the liver 96 h after intravenous application. Pilot fluorescence imaging confirmed the limited in vivo detection capabilities of the nanoparticles.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24013947
003      
CZ-PrNML
005      
20250521103743.0
007      
ta
008      
240725s2024 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms25105294 $2 doi
035    __
$a (PubMed)38791332
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Machová Urdzíková, Lucia $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic $1 https://orcid.org/0000000176225837 $7 xx0136406
245    10
$a Toxicity of Large and Small Surface-Engineered Upconverting Nanoparticles for In Vitro and In Vivo Bioapplications / $c L. Machová Urdzíková, D. Mareková, T. Vasylyshyn, P. Matouš, V. Patsula, V. Oleksa, O. Shapoval, M. Vosmanská, D. Liebl, A. Benda, V. Herynek, D. Horák, P. Jendelová
520    9_
$a In this study, spherical or hexagonal NaYF4:Yb,Er nanoparticles (UCNPs) with sizes of 25 nm (S-UCNPs) and 120 nm (L-UCNPs) were synthesized by high-temperature coprecipitation and subsequently modified with three kinds of polymers. These included poly(ethylene glycol) (PEG) and poly(N,N-dimethylacrylamide-co-2-aminoethylacrylamide) [P(DMA-AEA)] terminated with an alendronate anchoring group, and poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The internalization of nanoparticles by rat mesenchymal stem cells (rMSCs) and C6 cancer cells (rat glial tumor cell line) was visualized by electron microscopy and the cytotoxicity of the UCNPs and their leaches was measured by the real-time proliferation assay. The comet assay was used to determine the oxidative damage of the UCNPs. An in vivo study on mice determined the elimination route and potential accumulation of UCNPs in the body. The results showed that the L- and S-UCNPs were internalized into cells in the lumen of endosomes. The proliferation assay revealed that the L-UCNPs were less toxic than S-UCNPs. The viability of rMSCs incubated with particles decreased in the order S-UCNP@Ale-(PDMA-AEA) > S-UCNP@Ale-PEG > S-UCNPs > S-UCNP@PMVEMA. Similar results were obtained in C6 cells. The oxidative damage measured by the comet assay showed that neat L-UCNPs caused more oxidative damage to rMSCs than all coated UCNPs while no difference was observed in C6 cells. An in vivo study indicated that L-UCNPs were eliminated from the body via the hepatobiliary route; L-UCNP@Ale-PEG particles were almost eliminated from the liver 96 h after intravenous application. Pilot fluorescence imaging confirmed the limited in vivo detection capabilities of the nanoparticles.
650    _2
$a zvířata $7 D000818
650    _2
$a myši $7 D051379
650    _2
$a krysa rodu Rattus $7 D051381
650    12
$a mezenchymální kmenové buňky $x metabolismus $x účinky léků $x cytologie $7 D059630
650    _2
$a nanočástice $x chemie $7 D053758
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a polyethylenglykoly $x chemie $7 D011092
650    _2
$a viabilita buněk $x účinky léků $7 D002470
650    _2
$a velikost částic $7 D010316
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a oxidační stres $x účinky léků $7 D018384
655    _2
$a časopisecké články $7 D016428
700    1_
$a Mareková, Dana $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic
700    1_
$a Vasylyshyn, Taras $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 16200 Prague, Czech Republic $1 https://orcid.org/0000000237625624
700    1_
$a Matouš, Petr $u Center for Advanced Preclinical Imaging, First Faculty of Medicine, Charles University, Salmovská 3, 12000 Prague, Czech Republic $1 https://orcid.org/0000000293535842 $7 xx0332461
700    1_
$a Patsula, Vitalii $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 16200 Prague, Czech Republic
700    1_
$a Oleksa, Viktoriia $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 16200 Prague, Czech Republic
700    1_
$a Shapoval, Oleksandr $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 16200 Prague, Czech Republic $1 https://orcid.org/0000000205886256
700    1_
$a Vosmanská, Magda $u Department of Analytical Chemistry, University of Chemistry and Technology, Technická 5, 16000 Prague, Czech Republic $1 https://orcid.org/0000000294038688
700    1_
$a Liebl, David $u Imaging Methods Core Facility, BIOCEV, Faculty of Science, Charles University, Průmyslová 595, 25250 Vestec-Jesenice u Prahy, Czech Republic
700    1_
$a Benda, Aleš $u Imaging Methods Core Facility, BIOCEV, Faculty of Science, Charles University, Průmyslová 595, 25250 Vestec-Jesenice u Prahy, Czech Republic $1 https://orcid.org/0000000158298362
700    1_
$a Herynek, Vít $u Center for Advanced Preclinical Imaging, First Faculty of Medicine, Charles University, Salmovská 3, 12000 Prague, Czech Republic $1 https://orcid.org/0000000217752394 $7 xx0069420
700    1_
$a Horák, Daniel $u Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 16200 Prague, Czech Republic $1 https://orcid.org/0000000269079701 $7 xx0076519
700    1_
$a Jendelová, Pavla $u Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic $1 https://orcid.org/0000000246449212 $7 xx0068971
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 25, č. 10 (2024)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38791332 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240725 $b ABA008
991    __
$a 20250521103741 $b ABA008
999    __
$a ok $b bmc $g 2143636 $s 1225813
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 25 $c 10 $e 20240513 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$a OPJAK CZ.02.01.01/00/22_008/0004562 $p Ministry of sports and education
GRA    __
$a No. 21-04420S $p Czech Science Foundation
LZP    __
$a Pubmed-20240725
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace