Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Majority of Treponema pallidum ssp. pallidum MLST allelic profiles in the Czech Republic (2004-2022) belong to two SS14-like clusters

E. Vrbová, P. Pospíšilová, E. Dastychová, M. Kojanová, M. Kreidlová, F. Rob, V. Vašků, P. Mosio, R. Strnadel, O. Faustmannová, I. Kuklová, MD. Heroldová, H. Zákoucká, D. Šmajs

. 2024 ; 14 (1) : 17463. [pub] 20240729

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24019665

Grant support
U19 AI144177 NIAID NIH HHS - United States
LX22NPO5103 National Institute of Virology and Bacteriology , Programme EXCELES
65269705 Ministerstvo Zdravotnictví Ceské Republiky
U19AI144177 US National Institutes of Health National Institute for Allergy and Infectious Disease

Syphilis is a multistage sexually transmitted disease caused by Treponema pallidum ssp. pallidum. In the Czech Republic, there are around 700-800 new syphilis cases annually, continuously increasing since 2012. This study analyzed a total of 1228 samples from 2004 to 2022. Of the PCR-positive typeable samples (n = 415), 68.7% were fully-typed (FT), and 31.3% were partially-typed. Most of the identified isolates belonged to the SS14-clade and only 6.3% were the Nichols-like cluster. While in the beginning of sample collection isolates have been macrolide-susceptible, recent isolates are completely resistant to macrolides. Among the FT samples, 34 different allelic profiles (APs) were found. Most of the profiles (n = 27) appeared just once in the Czech population, while seven profiles were detected more than twice. The most frequent APs belonged to two separate groups of SS14-like isolates, including group of 1.3.1 (ST 1) and 1.26.1 (ST 25) profiles, and the second group containing 1.1.8 (ST 3), 1.1.1 (ST 2), and 1.1.3 (ST 11) (representing 57.5%, and 25.3% of all detected APs, respectively). Both groups consistently differed in 6 nucleotide positions in five genes (TP0150, TP0324, TP0515, TP0548, and TP0691) coding amino-acid replacements suggesting that one or more of these differences could be involved in the higher success of the first group.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24019665
003      
CZ-PrNML
005      
20241024110714.0
007      
ta
008      
241015s2024 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-024-68656-5 $2 doi
035    __
$a (PubMed)39075238
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Vrbová, Eliška $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
245    10
$a Majority of Treponema pallidum ssp. pallidum MLST allelic profiles in the Czech Republic (2004-2022) belong to two SS14-like clusters / $c E. Vrbová, P. Pospíšilová, E. Dastychová, M. Kojanová, M. Kreidlová, F. Rob, V. Vašků, P. Mosio, R. Strnadel, O. Faustmannová, I. Kuklová, MD. Heroldová, H. Zákoucká, D. Šmajs
520    9_
$a Syphilis is a multistage sexually transmitted disease caused by Treponema pallidum ssp. pallidum. In the Czech Republic, there are around 700-800 new syphilis cases annually, continuously increasing since 2012. This study analyzed a total of 1228 samples from 2004 to 2022. Of the PCR-positive typeable samples (n = 415), 68.7% were fully-typed (FT), and 31.3% were partially-typed. Most of the identified isolates belonged to the SS14-clade and only 6.3% were the Nichols-like cluster. While in the beginning of sample collection isolates have been macrolide-susceptible, recent isolates are completely resistant to macrolides. Among the FT samples, 34 different allelic profiles (APs) were found. Most of the profiles (n = 27) appeared just once in the Czech population, while seven profiles were detected more than twice. The most frequent APs belonged to two separate groups of SS14-like isolates, including group of 1.3.1 (ST 1) and 1.26.1 (ST 25) profiles, and the second group containing 1.1.8 (ST 3), 1.1.1 (ST 2), and 1.1.3 (ST 11) (representing 57.5%, and 25.3% of all detected APs, respectively). Both groups consistently differed in 6 nucleotide positions in five genes (TP0150, TP0324, TP0515, TP0548, and TP0691) coding amino-acid replacements suggesting that one or more of these differences could be involved in the higher success of the first group.
650    _2
$a lidé $7 D006801
650    12
$a Treponema pallidum $x genetika $x izolace a purifikace $7 D014210
650    12
$a syfilis $x mikrobiologie $x epidemiologie $x genetika $7 D013587
650    12
$a alely $7 D000483
650    12
$a multilokusová sekvenční typizace $7 D058885
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a dospělí $7 D000328
650    _2
$a makrolidy $x farmakologie $7 D018942
650    _2
$a lidé středního věku $7 D008875
650    _2
$a genotyp $7 D005838
651    _2
$a Česká republika $7 D018153
655    _2
$a časopisecké články $7 D016428
700    1_
$a Pospíšilová, Petra $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Dastychová, Eliška $u First Department of Dermatovenereology, St. Annes Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Kojanová, Martina $u Department of Dermatovenereology, First Faculty of Medicine and General University Hospital, Charles University, Prague, Czech Republic
700    1_
$a Kreidlová, Miluše $u Institute of Medical Biochemistry and Laboratory Diagnostics of the General University Hospital and of the First Faculty of Medicine of Charles University, Prague, Czech Republic
700    1_
$a Rob, Filip $u Department of Dermatovenerology, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
700    1_
$a Vašků, Vladimír $u First Department of Dermatovenereology, St. Annes Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic
700    1_
$a Mosio, Petra $u National Reference Laboratory for Diagnostics of Syphilis, National Institute for Public Health, Prague, Czech Republic
700    1_
$a Strnadel, Radim $u Department of Dermatovenerology, University Hospital, Brno, Czech Republic
700    1_
$a Faustmannová, Olga $u Department of Dermatovenerology, University Hospital, Brno, Czech Republic
700    1_
$a Kuklová, Ivana $u Department of Dermatovenereology, First Faculty of Medicine and General University Hospital, Charles University, Prague, Czech Republic
700    1_
$a Heroldová, Monika Dvořáková $u Department of Medical Microbiology, Faculty of Medicine, St. Anne's Hospital and Masaryk University, Brno, Czech Republic
700    1_
$a Zákoucká, Hana $u National Reference Laboratory for Diagnostics of Syphilis, National Institute for Public Health, Prague, Czech Republic
700    1_
$a Šmajs, David $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. dsmajs@med.muni.cz
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 14, č. 1 (2024), s. 17463
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39075238 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20241015 $b ABA008
991    __
$a 20241024110708 $b ABA008
999    __
$a ok $b bmc $g 2202102 $s 1231638
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 14 $c 1 $d 17463 $e 20240729 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
GRA    __
$a U19 AI144177 $p NIAID NIH HHS $2 United States
GRA    __
$a LX22NPO5103 $p National Institute of Virology and Bacteriology , Programme EXCELES
GRA    __
$a 65269705 $p Ministerstvo Zdravotnictví Ceské Republiky
GRA    __
$a U19AI144177 $p US National Institutes of Health National Institute for Allergy and Infectious Disease
LZP    __
$a Pubmed-20241015

Find record

Citation metrics

Archiving options