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Complexity of synovial fluid-derived monocyte-macrophage-lineage cells in knee osteoarthritis
Z. Mikulkova, J. Gallo, G. Manukyan, M. Trajerova, J. Savara, B. Shrestha, T. Dyskova, R. Nesnadna, Z. Slobodova, M. Stefancik, E. Kriegova
Language English Country United States
Document type Journal Article
NLK
Cell Press Free Archives
from 2012
Directory of Open Access Journals
from 2012
Free Medical Journals
from 2012
Freely Accessible Science Journals
from 2012-01-26
Open Access Digital Library
from 2012-01-26
Open Access Digital Library
from 2012-01-01
- MeSH
- Osteoarthritis, Knee * pathology metabolism immunology MeSH
- Cell Lineage MeSH
- Dendritic Cells * metabolism immunology MeSH
- Middle Aged MeSH
- Humans MeSH
- Macrophages * metabolism immunology MeSH
- Monocytes * metabolism immunology MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Synovial Fluid * metabolism immunology MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
Synovial fluid (SF)-derived monocyte-macrophage (MON-Mφ)-lineage cells in knee osteoarthritis (KOA) remain poorly understood. We analyzed SF samples from 420 patients with KOA with effusion. The MON-Mφ cells accounted for 47.4% (median; range 7.1%-94.4%) of CD45+ cells and consisted of four subpopulations that correlated with the distribution and activation of other immune cells. The most abundant subpopulation was that of inactive CD11b+CD14-CD16- myeloid dendritic cells (mDCs; cDC2), which exhibited low cytokine production, low T lymphocyte stimulation, and high migratory ability. Other major subpopulations included CD11b+CD14+CD16- monocyte-like cells and CD11b+CD14+CD16+ macrophages, which share a similar transcriptomic profile. A subpopulation of CD11b-CD14-CD16- mDCs (cDC1) was less common. A higher proportion of CD11b+CD14-CD16- mDCs was linked to early-stage KOA and mild joint pain. Dendritic cells were rarely present in KOA synovium. This study revealed the considerable complexity of SF-derived MON-Mφ subpopulations and highlighted the role of inactive mDCs in KOA.
Department of Clinical and Molecular Pathology University Hospital Olomouc Olomouc Czechia
Department of Immunology University Hospital Olomouc Olomouc Czechia
Department of Orthopedics University Hospital Olomouc Olomouc Czechia
References provided by Crossref.org
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- $a Mikulkova, Zuzana $u Department of Immunology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czechia; Department of Immunology, University Hospital Olomouc, Olomouc, Czechia
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- $a Synovial fluid (SF)-derived monocyte-macrophage (MON-Mφ)-lineage cells in knee osteoarthritis (KOA) remain poorly understood. We analyzed SF samples from 420 patients with KOA with effusion. The MON-Mφ cells accounted for 47.4% (median; range 7.1%-94.4%) of CD45+ cells and consisted of four subpopulations that correlated with the distribution and activation of other immune cells. The most abundant subpopulation was that of inactive CD11b+CD14-CD16- myeloid dendritic cells (mDCs; cDC2), which exhibited low cytokine production, low T lymphocyte stimulation, and high migratory ability. Other major subpopulations included CD11b+CD14+CD16- monocyte-like cells and CD11b+CD14+CD16+ macrophages, which share a similar transcriptomic profile. A subpopulation of CD11b-CD14-CD16- mDCs (cDC1) was less common. A higher proportion of CD11b+CD14-CD16- mDCs was linked to early-stage KOA and mild joint pain. Dendritic cells were rarely present in KOA synovium. This study revealed the considerable complexity of SF-derived MON-Mφ subpopulations and highlighted the role of inactive mDCs in KOA.
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