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Complexity of synovial fluid-derived monocyte-macrophage-lineage cells in knee osteoarthritis
Z. Mikulkova, J. Gallo, G. Manukyan, M. Trajerova, J. Savara, B. Shrestha, T. Dyskova, R. Nesnadna, Z. Slobodova, M. Stefancik, E. Kriegova
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
NLK
Cell Press Free Archives
od 2012
Directory of Open Access Journals
od 2012
Free Medical Journals
od 2012
Freely Accessible Science Journals
od 2012-01-26
Open Access Digital Library
od 2012-01-26
Open Access Digital Library
od 2012-01-01
- MeSH
- artróza kolenních kloubů * patologie metabolismus imunologie MeSH
- buněčný rodokmen MeSH
- dendritické buňky * metabolismus imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- makrofágy * metabolismus imunologie MeSH
- monocyty * metabolismus imunologie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- synoviální tekutina * metabolismus imunologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Synovial fluid (SF)-derived monocyte-macrophage (MON-Mφ)-lineage cells in knee osteoarthritis (KOA) remain poorly understood. We analyzed SF samples from 420 patients with KOA with effusion. The MON-Mφ cells accounted for 47.4% (median; range 7.1%-94.4%) of CD45+ cells and consisted of four subpopulations that correlated with the distribution and activation of other immune cells. The most abundant subpopulation was that of inactive CD11b+CD14-CD16- myeloid dendritic cells (mDCs; cDC2), which exhibited low cytokine production, low T lymphocyte stimulation, and high migratory ability. Other major subpopulations included CD11b+CD14+CD16- monocyte-like cells and CD11b+CD14+CD16+ macrophages, which share a similar transcriptomic profile. A subpopulation of CD11b-CD14-CD16- mDCs (cDC1) was less common. A higher proportion of CD11b+CD14-CD16- mDCs was linked to early-stage KOA and mild joint pain. Dendritic cells were rarely present in KOA synovium. This study revealed the considerable complexity of SF-derived MON-Mφ subpopulations and highlighted the role of inactive mDCs in KOA.
Department of Clinical and Molecular Pathology University Hospital Olomouc Olomouc Czechia
Department of Immunology University Hospital Olomouc Olomouc Czechia
Department of Orthopedics University Hospital Olomouc Olomouc Czechia
Citace poskytuje Crossref.org
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- $a Mikulkova, Zuzana $u Department of Immunology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czechia; Department of Immunology, University Hospital Olomouc, Olomouc, Czechia
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- $a Synovial fluid (SF)-derived monocyte-macrophage (MON-Mφ)-lineage cells in knee osteoarthritis (KOA) remain poorly understood. We analyzed SF samples from 420 patients with KOA with effusion. The MON-Mφ cells accounted for 47.4% (median; range 7.1%-94.4%) of CD45+ cells and consisted of four subpopulations that correlated with the distribution and activation of other immune cells. The most abundant subpopulation was that of inactive CD11b+CD14-CD16- myeloid dendritic cells (mDCs; cDC2), which exhibited low cytokine production, low T lymphocyte stimulation, and high migratory ability. Other major subpopulations included CD11b+CD14+CD16- monocyte-like cells and CD11b+CD14+CD16+ macrophages, which share a similar transcriptomic profile. A subpopulation of CD11b-CD14-CD16- mDCs (cDC1) was less common. A higher proportion of CD11b+CD14-CD16- mDCs was linked to early-stage KOA and mild joint pain. Dendritic cells were rarely present in KOA synovium. This study revealed the considerable complexity of SF-derived MON-Mφ subpopulations and highlighted the role of inactive mDCs in KOA.
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- $a Manukyan, Gayane $u Department of Immunology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czechia; Department of Immunology, University Hospital Olomouc, Olomouc, Czechia; Laboratory of Molecular and Cellular Immunology, Institute of Molecular Biology NAS RA, Yerevan, Armenia
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- $a Kriegova, Eva $u Department of Immunology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czechia; Department of Immunology, University Hospital Olomouc, Olomouc, Czechia. Electronic address: eva.kriegova@email.cz
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