• Je něco špatně v tomto záznamu ?

Optimizing Subretinal Bleb Formation for Visual Streak Involvement in a Porcine Model for Retinal Gene Therapy

IP. Seitz, T. Peters, F. Reichel, A. Bähr, H. Auch, J. Motlik, T. Ardan, J. Juhasova, Y. Nemesh, S. Juhás, N. Klymiuk, MD. Fischer

. 2024 ; 65 (12) : 41. [pub] 20241001

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25003966

PURPOSE: Subretinal (SR) injection in porcine models is a promising avenue for preclinical evaluation of cell and gene therapies. Targeting of the subretinal fluid compartment (bleb) is critical to the procedure, especially if treatment of the cone-rich area centralis is required (i.e., visual streak [VS] in pigs). To our knowledge, this study is the first to investigate the influence of injection site placement on VS involvement in the pig eye. METHODS: We performed 23-gauge pars plana vitrectomy followed by SR injection in 41 eyes of 21 animals (Sus scrofa domesticus). In 27 eyes (65.9%), the injection site was placed superior to the VS, and in 14 eyes (34.1%) it was placed inferior to it. Using intraoperative imaging, blebs were classified based on their propagation behavior relative to the VS. RESULTS: In 79% of cases, blebs from inferior injection sites developed away from the VS, exhibiting a mean ± SEM vertical anisotropy (AP) of 0.67 ± 0.11. In contrast, blebs from superior injection sites tended to develop toward the VS with an AP of 1.27 ± 0.18 (P = 0.0070). Blebs developed away from the VS in only 41% of injections (P = 0.0212). Inferior blebs were orientated close to 0° (horizontal), whereas superior blebs displayed varied orientations with a mean angle of 56° (P = 0.0008). CONCLUSIONS: Bleb propagation was anisotropic (i.e., directionally biased) and dependent on injection site placement. Superior injection sites led to superior VS detachment. Morphological analysis suggested increased adhesion forces at the VS and superior vascular arcades. This study will aid the planning of surgeries for targeted subretinal delivery in pig models.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25003966
003      
CZ-PrNML
005      
20250206104827.0
007      
ta
008      
250121s2024 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1167/iovs.65.12.41 $2 doi
035    __
$a (PubMed)39466231
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Seitz, Immanuel P $u University Eye Hospital, Eberhard-Karls-Universität Tübingen, Tübingen, Baden-Württemberg, Germany
245    10
$a Optimizing Subretinal Bleb Formation for Visual Streak Involvement in a Porcine Model for Retinal Gene Therapy / $c IP. Seitz, T. Peters, F. Reichel, A. Bähr, H. Auch, J. Motlik, T. Ardan, J. Juhasova, Y. Nemesh, S. Juhás, N. Klymiuk, MD. Fischer
520    9_
$a PURPOSE: Subretinal (SR) injection in porcine models is a promising avenue for preclinical evaluation of cell and gene therapies. Targeting of the subretinal fluid compartment (bleb) is critical to the procedure, especially if treatment of the cone-rich area centralis is required (i.e., visual streak [VS] in pigs). To our knowledge, this study is the first to investigate the influence of injection site placement on VS involvement in the pig eye. METHODS: We performed 23-gauge pars plana vitrectomy followed by SR injection in 41 eyes of 21 animals (Sus scrofa domesticus). In 27 eyes (65.9%), the injection site was placed superior to the VS, and in 14 eyes (34.1%) it was placed inferior to it. Using intraoperative imaging, blebs were classified based on their propagation behavior relative to the VS. RESULTS: In 79% of cases, blebs from inferior injection sites developed away from the VS, exhibiting a mean ± SEM vertical anisotropy (AP) of 0.67 ± 0.11. In contrast, blebs from superior injection sites tended to develop toward the VS with an AP of 1.27 ± 0.18 (P = 0.0070). Blebs developed away from the VS in only 41% of injections (P = 0.0212). Inferior blebs were orientated close to 0° (horizontal), whereas superior blebs displayed varied orientations with a mean angle of 56° (P = 0.0008). CONCLUSIONS: Bleb propagation was anisotropic (i.e., directionally biased) and dependent on injection site placement. Superior injection sites led to superior VS detachment. Morphological analysis suggested increased adhesion forces at the VS and superior vascular arcades. This study will aid the planning of surgeries for targeted subretinal delivery in pig models.
650    _2
$a zvířata $7 D000818
650    12
$a genetická terapie $x metody $7 D015316
650    12
$a vitrektomie $x metody $7 D014821
650    _2
$a prasata $7 D013552
650    12
$a modely nemocí na zvířatech $7 D004195
650    _2
$a retina $7 D012160
650    _2
$a subretinální tekutina $x metabolismus $7 D058471
650    _2
$a injekce nitrooční $7 D056965
650    _2
$a Sus scrofa $7 D034421
655    _2
$a časopisecké články $7 D016428
700    1_
$a Peters, Tobias $u University Eye Hospital, Eberhard-Karls-Universität Tübingen, Tübingen, Baden-Württemberg, Germany
700    1_
$a Reichel, Felix $u University Eye Hospital, Eberhard-Karls-Universität Tübingen, Tübingen, Baden-Württemberg, Germany $u Oxford University Hospitals NHS Foundation Trust, Oxford Eye Hospital, Oxford, Oxfordshire, United Kingdom
700    1_
$a Bähr, Andrea $u Medical Department I, Cardiology, Angiology, Pneumology, Klinikum rechts der Isar, Technische Universität München, München, Bayern, Germany $u Center for Innovative Medical Models, Ludwig-Maximilians-Universität München, München, Bayern, Germany
700    1_
$a Auch, Hanna $u Medical Department I, Cardiology, Angiology, Pneumology, Klinikum rechts der Isar, Technische Universität München, München, Bayern, Germany $u Center for Innovative Medical Models, Ludwig-Maximilians-Universität München, München, Bayern, Germany
700    1_
$a Motlik, Jan $u Institute of Animal Physiology and Genetics, Akademie věd České republiky, Praha, Czechia
700    1_
$a Ardan, Taras $u Institute of Animal Physiology and Genetics, Akademie věd České republiky, Praha, Czechia
700    1_
$a Juhasova, Jana $u Institute of Animal Physiology and Genetics, Akademie věd České republiky, Praha, Czechia
700    1_
$a Nemesh, Yaroslav $u Institute of Animal Physiology and Genetics, Akademie věd České republiky, Praha, Czechia
700    1_
$a Juhás, Stefan $u Institute of Animal Physiology and Genetics, Akademie věd České republiky, Praha, Czechia
700    1_
$a Klymiuk, Nikolai $u Medical Department I, Cardiology, Angiology, Pneumology, Klinikum rechts der Isar, Technische Universität München, München, Bayern, Germany $u Center for Innovative Medical Models, Ludwig-Maximilians-Universität München, München, Bayern, Germany
700    1_
$a Fischer, Manuel Dominik $u University Eye Hospital, Eberhard-Karls-Universität Tübingen, Tübingen, Baden-Württemberg, Germany $u Oxford University Hospitals NHS Foundation Trust, Oxford Eye Hospital, Oxford, Oxfordshire, United Kingdom $u Nuffield Department of Clinical Neurosciences, University of Oxford, The John Radcliffe Hospital, Oxford, United Kingdom
773    0_
$w MED00002404 $t Investigative ophthalmology & visual science $x 1552-5783 $g Roč. 65, č. 12 (2024), s. 41
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39466231 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250121 $b ABA008
991    __
$a 20250206104823 $b ABA008
999    __
$a ok $b bmc $g 2263609 $s 1239973
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 65 $c 12 $d 41 $e 20241001 $i 1552-5783 $m Investigative ophthalmology & visual science $n Invest Ophthalmol Vis Sci $x MED00002404
LZP    __
$a Pubmed-20250121

Najít záznam

Citační ukazatele

Možnosti archivace