Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

The immune response modulated by inoculation of commensal bacteria at birth impacts the gut microbiota and prevents Salmonella colonization

F. Kempf, R. Drumo, AM. Chaussé, P. Menanteau, T. Kubasova, S. Roche, AC. Lalmanach, R. Guabiraba, T. Chaumeil, G. Larivière-Gauthier, I. Caballero-Posadas, B. Laroche, I. Rychlík, I. Virlogeux-Payant, P. Velge

. 2025 ; 17 (1) : 2474151. [pub] 20250313

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc25009254

Super- and low-shedding phenomena have been observed in genetically homogeneous hosts infected by a single bacterial strain. To decipher the mechanisms underlying these phenotypes, we conducted an experiment with chicks infected with Salmonella Enteritidis in a non-sterile isolator, which prevents bacterial transmission between animals while allowing the development of the gut microbiota. We investigated the impact of four commensal bacteria called Mix4, inoculated at hatching, on chicken systemic immune response and intestinal microbiota composition and functions, before and after Salmonella infection. Our results revealed that these phenotypes were not linked to changes in cell invasion capacity of bacteria during infection. Mix4 inoculation had both short- and long-term effects on immune response and microbiota and promoted the low-shedder phenotype. Kinetic analysis revealed that Mix4 activated immune response from day 4, which modified the microbiota on day 6. This change promotes a more fermentative microbiota, using the aromatic compounds degradation pathway, which inhibited Salmonella colonization by day 11 and beyond. In contrast, control animals exhibited a delayed TNF-driven pro-inflammatory response and developed a microbiota using anaerobic respiration, which facilitates Salmonella colonization and growth. This strategy offers promising opportunities to strengthen the barrier effect against Salmonella and possibly other pathogens.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25009254
003      
CZ-PrNML
005      
20250429135200.0
007      
ta
008      
250415s2025 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1080/19490976.2025.2474151 $2 doi
035    __
$a (PubMed)40079593
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kempf, Florent $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France $1 https://orcid.org/0000000288013601
245    14
$a The immune response modulated by inoculation of commensal bacteria at birth impacts the gut microbiota and prevents Salmonella colonization / $c F. Kempf, R. Drumo, AM. Chaussé, P. Menanteau, T. Kubasova, S. Roche, AC. Lalmanach, R. Guabiraba, T. Chaumeil, G. Larivière-Gauthier, I. Caballero-Posadas, B. Laroche, I. Rychlík, I. Virlogeux-Payant, P. Velge
520    9_
$a Super- and low-shedding phenomena have been observed in genetically homogeneous hosts infected by a single bacterial strain. To decipher the mechanisms underlying these phenotypes, we conducted an experiment with chicks infected with Salmonella Enteritidis in a non-sterile isolator, which prevents bacterial transmission between animals while allowing the development of the gut microbiota. We investigated the impact of four commensal bacteria called Mix4, inoculated at hatching, on chicken systemic immune response and intestinal microbiota composition and functions, before and after Salmonella infection. Our results revealed that these phenotypes were not linked to changes in cell invasion capacity of bacteria during infection. Mix4 inoculation had both short- and long-term effects on immune response and microbiota and promoted the low-shedder phenotype. Kinetic analysis revealed that Mix4 activated immune response from day 4, which modified the microbiota on day 6. This change promotes a more fermentative microbiota, using the aromatic compounds degradation pathway, which inhibited Salmonella colonization by day 11 and beyond. In contrast, control animals exhibited a delayed TNF-driven pro-inflammatory response and developed a microbiota using anaerobic respiration, which facilitates Salmonella colonization and growth. This strategy offers promising opportunities to strengthen the barrier effect against Salmonella and possibly other pathogens.
650    _2
$a zvířata $7 D000818
650    12
$a střevní mikroflóra $7 D000069196
650    12
$a kur domácí $x mikrobiologie $x imunologie $7 D002645
650    12
$a Salmonella enteritidis $x imunologie $x růst a vývoj $7 D012477
650    12
$a salmonelová infekce u zvířat $x mikrobiologie $x imunologie $x prevence a kontrola $7 D012481
650    12
$a Bacteria $x klasifikace $x genetika $x izolace a purifikace $7 D001419
650    12
$a nemoci drůbeže $x mikrobiologie $x imunologie $x prevence a kontrola $7 D011201
650    _2
$a symbióza $7 D013559
655    _2
$a časopisecké články $7 D016428
700    1_
$a Drumo, Rosanna $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Chaussé, Anne Marie $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Menanteau, Pierrette $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Kubasova, Tereza $u Department of Microbiology and antimicrobial resistance, Veterinary Research Institute, Brno, Czech Republic
700    1_
$a Roche, Sylvie $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Lalmanach, Anne Christine $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Guabiraba, Rodrigo $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Chaumeil, Thierry $u Plate-Forme d'Infectiologie Expérimentale, INRAE, Nouzilly, France
700    1_
$a Larivière-Gauthier, Guillaume $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Caballero-Posadas, Ignacio $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Laroche, Béatrice $u MaIAGE, INRAE, Jouy-en-Josas, France
700    1_
$a Rychlík, Ivan $u Department of Microbiology and antimicrobial resistance, Veterinary Research Institute, Brno, Czech Republic
700    1_
$a Virlogeux-Payant, Isabelle $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
700    1_
$a Velge, Philippe $u ISP, INRAE, Université François Rabelais de Tours, Nouzilly, France
773    0_
$w MED00175586 $t Gut microbes $x 1949-0984 $g Roč. 17, č. 1 (2025), s. 2474151
856    41
$u https://pubmed.ncbi.nlm.nih.gov/40079593 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250415 $b ABA008
991    __
$a 20250429135156 $b ABA008
999    __
$a ok $b bmc $g 2310941 $s 1246335
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 17 $c 1 $d 2474151 $e 20250313 $i 1949-0984 $m Gut microbes $n Gut Microbes $x MED00175586
LZP    __
$a Pubmed-20250415

Find record

Citation metrics

Archiving options