-
Something wrong with this record ?
Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens
E. Ferreras-Colino, J. de la Fuente, J. Couto, M. Golovchenko, S. Antunes, IA. Sevilla, A. Domingos, N. Rudenko, M. Contreras, R. Martínez-Camacho, C. Gortazar, MA. Risalde
Language English Country Netherlands
Document type Journal Article
NLK
ProQuest Central
from 2002-01-01 to 2 months ago
Nursing & Allied Health Database (ProQuest)
from 2002-01-01 to 2 months ago
Health & Medicine (ProQuest)
from 2002-01-01 to 2 months ago
Family Health Database (ProQuest)
from 2002-01-01 to 2 months ago
Health Management Database (ProQuest)
from 2002-01-01 to 2 months ago
Public Health Database (ProQuest)
from 2002-01-01 to 2 months ago
- MeSH
- Adjuvants, Immunologic * administration & dosage MeSH
- BCG Vaccine * immunology administration & dosage MeSH
- Borrelia burgdorferi immunology MeSH
- Cytokines MeSH
- Vaccines, Inactivated immunology administration & dosage MeSH
- Interferon-gamma immunology MeSH
- Interleukin-1alpha immunology MeSH
- Liver immunology MeSH
- Lyme Disease * prevention & control immunology MeSH
- Macrophages immunology MeSH
- Malaria * prevention & control immunology MeSH
- Mycobacterium bovis * immunology MeSH
- Mice, Inbred BALB C MeSH
- Mice, Inbred C3H MeSH
- Mice MeSH
- Plasmodium berghei immunology MeSH
- Antibodies, Bacterial blood MeSH
- Spleen immunology microbiology MeSH
- Tumor Necrosis Factor-alpha immunology MeSH
- Hot Temperature MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens. Bacillus Calmette-Guérin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved. BALB/c and C3H/HeN mice were randomly assigned in similar number to either immunized group receiving two oral doses of HIMB with a 4-week interval, or control group treated with PBS. All the BALB/c mice were intraperitoneally infected with P. berghei while the C3H/HeN mice were subcutaneously infected with B. burgdorferi. Pathogen burden was significantly reduced in both immunized groups when compared to controls. The number of macrophages significantly decreased in the liver or in the spleen of the mice that had been immunized prior to the challenge with P. berghei or B. burgdorferi, respectively. Furthermore, the immunized groups showed an apparent upregulation of IFN-γ, TNF-α and IL-1α in the liver (P. berghei challenge) or a significant increase in IL-1α producing cells in the spleen (B. burgdorferi challenge). Our findings suggest that oral immunization with heat-inactivated mycobacteria limits pathogen burden through stimulation of the innate immune response in two vector-borne diseases in mice.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc25016075
- 003
- CZ-PrNML
- 005
- 20250731091506.0
- 007
- ta
- 008
- 250708e20250405ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.vaccine.2025.127076 $2 doi
- 035 __
- $a (PubMed)40188566
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Ferreras-Colino, Elisa $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain
- 245 10
- $a Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens / $c E. Ferreras-Colino, J. de la Fuente, J. Couto, M. Golovchenko, S. Antunes, IA. Sevilla, A. Domingos, N. Rudenko, M. Contreras, R. Martínez-Camacho, C. Gortazar, MA. Risalde
- 520 9_
- $a Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens. Bacillus Calmette-Guérin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved. BALB/c and C3H/HeN mice were randomly assigned in similar number to either immunized group receiving two oral doses of HIMB with a 4-week interval, or control group treated with PBS. All the BALB/c mice were intraperitoneally infected with P. berghei while the C3H/HeN mice were subcutaneously infected with B. burgdorferi. Pathogen burden was significantly reduced in both immunized groups when compared to controls. The number of macrophages significantly decreased in the liver or in the spleen of the mice that had been immunized prior to the challenge with P. berghei or B. burgdorferi, respectively. Furthermore, the immunized groups showed an apparent upregulation of IFN-γ, TNF-α and IL-1α in the liver (P. berghei challenge) or a significant increase in IL-1α producing cells in the spleen (B. burgdorferi challenge). Our findings suggest that oral immunization with heat-inactivated mycobacteria limits pathogen burden through stimulation of the innate immune response in two vector-borne diseases in mice.
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a Mycobacterium bovis $x imunologie $7 D009163
- 650 _2
- $a myši inbrední BALB C $7 D008807
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a slezina $x imunologie $x mikrobiologie $7 D013154
- 650 _2
- $a Plasmodium berghei $x imunologie $7 D010962
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a myši inbrední C3H $7 D008809
- 650 _2
- $a Borrelia burgdorferi $x imunologie $7 D025065
- 650 _2
- $a játra $x imunologie $7 D008099
- 650 _2
- $a inaktivované vakcíny $x imunologie $x aplikace a dávkování $7 D015164
- 650 12
- $a lymeská nemoc $x prevence a kontrola $x imunologie $7 D008193
- 650 12
- $a malárie $x prevence a kontrola $x imunologie $7 D008288
- 650 _2
- $a makrofágy $x imunologie $7 D008264
- 650 _2
- $a interferon gama $x imunologie $7 D007371
- 650 12
- $a adjuvancia imunologická $x aplikace a dávkování $7 D000276
- 650 _2
- $a interleukin-1alfa $x imunologie $7 D053582
- 650 _2
- $a TNF-alfa $x imunologie $7 D014409
- 650 _2
- $a protilátky bakteriální $x krev $7 D000907
- 650 12
- $a BCG vakcína $x imunologie $x aplikace a dávkování $7 D001500
- 650 _2
- $a vysoká teplota $7 D006358
- 650 _2
- $a cytokiny $7 D016207
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a de la Fuente, José $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
- 700 1_
- $a Couto, Joana $u Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM/IHMT NOVA), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal; Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal
- 700 1_
- $a Golovchenko, Maryna $u Biology Centre Czech Academy of Sciences, Institute of Parasitology, Branisovska 31, 37005, Ceske Budejovice, Czech Republic
- 700 1_
- $a Antunes, Sandra $u Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM/IHMT NOVA), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal; Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal
- 700 1_
- $a Sevilla, Iker A $u NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Animal Health Department, Bizkaia Science and Technology Park 812L, 48160 Derio (Bizkaia), Spain
- 700 1_
- $a Domingos, Ana $u Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM/IHMT NOVA), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal; Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal
- 700 1_
- $a Rudenko, Natalie $u Biology Centre Czech Academy of Sciences, Institute of Parasitology, Branisovska 31, 37005, Ceske Budejovice, Czech Republic
- 700 1_
- $a Contreras, Marinela $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain
- 700 1_
- $a Martínez-Camacho, Rafael $u Departamento de Anatomía y Anatomía Patológica Comparadas y Toxicología, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Campus de Rabanales, Edificio Sanidad Animal, 14014, Córdoba, Spain
- 700 1_
- $a Gortazar, Christian $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain. Electronic address: christian.gortazar@uclm.es
- 700 1_
- $a Risalde, María A $u Departamento de Anatomía y Anatomía Patológica Comparadas y Toxicología, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Campus de Rabanales, Edificio Sanidad Animal, 14014, Córdoba, Spain; CIBERINFEC, ISCIII - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain
- 773 0_
- $w MED00004631 $t Vaccine $x 1873-2518 $g Roč. 53 (20250405), s. 127076
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/40188566 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20250708 $b ABA008
- 991 __
- $a 20250731091501 $b ABA008
- 999 __
- $a ok $b bmc $g 2366728 $s 1253200
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2025 $b 53 $c - $d 127076 $e 20250405 $i 1873-2518 $m Vaccine $n Vaccine $x MED00004631
- LZP __
- $a Pubmed-20250708
