Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens

E. Ferreras-Colino, J. de la Fuente, J. Couto, M. Golovchenko, S. Antunes, IA. Sevilla, A. Domingos, N. Rudenko, M. Contreras, R. Martínez-Camacho, C. Gortazar, MA. Risalde

. 2025 ; 53 (-) : 127076. [pub] 20250405

Language English Country Netherlands

Document type Journal Article

E-resources Online Full text

NLK ProQuest Central from 2002-01-01 to 2 months ago
Nursing & Allied Health Database (ProQuest) from 2002-01-01 to 2 months ago
Health & Medicine (ProQuest) from 2002-01-01 to 2 months ago
Family Health Database (ProQuest) from 2002-01-01 to 2 months ago
Health Management Database (ProQuest) from 2002-01-01 to 2 months ago
Public Health Database (ProQuest) from 2002-01-01 to 2 months ago

Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens. Bacillus Calmette-Guérin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved. BALB/c and C3H/HeN mice were randomly assigned in similar number to either immunized group receiving two oral doses of HIMB with a 4-week interval, or control group treated with PBS. All the BALB/c mice were intraperitoneally infected with P. berghei while the C3H/HeN mice were subcutaneously infected with B. burgdorferi. Pathogen burden was significantly reduced in both immunized groups when compared to controls. The number of macrophages significantly decreased in the liver or in the spleen of the mice that had been immunized prior to the challenge with P. berghei or B. burgdorferi, respectively. Furthermore, the immunized groups showed an apparent upregulation of IFN-γ, TNF-α and IL-1α in the liver (P. berghei challenge) or a significant increase in IL-1α producing cells in the spleen (B. burgdorferi challenge). Our findings suggest that oral immunization with heat-inactivated mycobacteria limits pathogen burden through stimulation of the innate immune response in two vector-borne diseases in mice.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25016075
003      
CZ-PrNML
005      
20250731091506.0
007      
ta
008      
250708e20250405ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.vaccine.2025.127076 $2 doi
035    __
$a (PubMed)40188566
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Ferreras-Colino, Elisa $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain
245    10
$a Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens / $c E. Ferreras-Colino, J. de la Fuente, J. Couto, M. Golovchenko, S. Antunes, IA. Sevilla, A. Domingos, N. Rudenko, M. Contreras, R. Martínez-Camacho, C. Gortazar, MA. Risalde
520    9_
$a Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens. Bacillus Calmette-Guérin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved. BALB/c and C3H/HeN mice were randomly assigned in similar number to either immunized group receiving two oral doses of HIMB with a 4-week interval, or control group treated with PBS. All the BALB/c mice were intraperitoneally infected with P. berghei while the C3H/HeN mice were subcutaneously infected with B. burgdorferi. Pathogen burden was significantly reduced in both immunized groups when compared to controls. The number of macrophages significantly decreased in the liver or in the spleen of the mice that had been immunized prior to the challenge with P. berghei or B. burgdorferi, respectively. Furthermore, the immunized groups showed an apparent upregulation of IFN-γ, TNF-α and IL-1α in the liver (P. berghei challenge) or a significant increase in IL-1α producing cells in the spleen (B. burgdorferi challenge). Our findings suggest that oral immunization with heat-inactivated mycobacteria limits pathogen burden through stimulation of the innate immune response in two vector-borne diseases in mice.
650    _2
$a zvířata $7 D000818
650    12
$a Mycobacterium bovis $x imunologie $7 D009163
650    _2
$a myši inbrední BALB C $7 D008807
650    _2
$a myši $7 D051379
650    _2
$a slezina $x imunologie $x mikrobiologie $7 D013154
650    _2
$a Plasmodium berghei $x imunologie $7 D010962
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a myši inbrední C3H $7 D008809
650    _2
$a Borrelia burgdorferi $x imunologie $7 D025065
650    _2
$a játra $x imunologie $7 D008099
650    _2
$a inaktivované vakcíny $x imunologie $x aplikace a dávkování $7 D015164
650    12
$a lymeská nemoc $x prevence a kontrola $x imunologie $7 D008193
650    12
$a malárie $x prevence a kontrola $x imunologie $7 D008288
650    _2
$a makrofágy $x imunologie $7 D008264
650    _2
$a interferon gama $x imunologie $7 D007371
650    12
$a adjuvancia imunologická $x aplikace a dávkování $7 D000276
650    _2
$a interleukin-1alfa $x imunologie $7 D053582
650    _2
$a TNF-alfa $x imunologie $7 D014409
650    _2
$a protilátky bakteriální $x krev $7 D000907
650    12
$a BCG vakcína $x imunologie $x aplikace a dávkování $7 D001500
650    _2
$a vysoká teplota $7 D006358
650    _2
$a cytokiny $7 D016207
655    _2
$a časopisecké články $7 D016428
700    1_
$a de la Fuente, José $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
700    1_
$a Couto, Joana $u Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM/IHMT NOVA), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal; Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal
700    1_
$a Golovchenko, Maryna $u Biology Centre Czech Academy of Sciences, Institute of Parasitology, Branisovska 31, 37005, Ceske Budejovice, Czech Republic
700    1_
$a Antunes, Sandra $u Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM/IHMT NOVA), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal; Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal
700    1_
$a Sevilla, Iker A $u NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Animal Health Department, Bizkaia Science and Technology Park 812L, 48160 Derio (Bizkaia), Spain
700    1_
$a Domingos, Ana $u Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM/IHMT NOVA), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal; Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal
700    1_
$a Rudenko, Natalie $u Biology Centre Czech Academy of Sciences, Institute of Parasitology, Branisovska 31, 37005, Ceske Budejovice, Czech Republic
700    1_
$a Contreras, Marinela $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain
700    1_
$a Martínez-Camacho, Rafael $u Departamento de Anatomía y Anatomía Patológica Comparadas y Toxicología, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Campus de Rabanales, Edificio Sanidad Animal, 14014, Córdoba, Spain
700    1_
$a Gortazar, Christian $u SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (UCLM-CSIC), Ciudad Real, Spain. Electronic address: christian.gortazar@uclm.es
700    1_
$a Risalde, María A $u Departamento de Anatomía y Anatomía Patológica Comparadas y Toxicología, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Campus de Rabanales, Edificio Sanidad Animal, 14014, Córdoba, Spain; CIBERINFEC, ISCIII - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain
773    0_
$w MED00004631 $t Vaccine $x 1873-2518 $g Roč. 53 (20250405), s. 127076
856    41
$u https://pubmed.ncbi.nlm.nih.gov/40188566 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250708 $b ABA008
991    __
$a 20250731091501 $b ABA008
999    __
$a ok $b bmc $g 2366728 $s 1253200
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 53 $c - $d 127076 $e 20250405 $i 1873-2518 $m Vaccine $n Vaccine $x MED00004631
LZP    __
$a Pubmed-20250708

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...