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An In Vitro Tube Model for Cell Biocompatibility Study of Capping Materials for Regenerative Endodontics
N. Pilbauerova, D. Dasi, IJ. de Souza Araujo, GT. Huang
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
R21 DE029605
NIDCR NIH HHS - United States
- MeSH
- biokompatibilní materiály * MeSH
- fixní kombinace léků MeSH
- kultivované buňky MeSH
- lidé MeSH
- materiály pro překrytí zubní dřeně a pulpektomii * farmakologie MeSH
- oxidy farmakologie MeSH
- regenerativní endodoncie * metody MeSH
- silikáty farmakologie MeSH
- skloionomerní cementy farmakologie MeSH
- sloučeniny hliníku farmakologie MeSH
- sloučeniny vápníku farmakologie MeSH
- techniky in vitro MeSH
- testování materiálů MeSH
- zubní dřeň * cytologie účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
INTRODUCTION: Cell-based pulp regeneration utilizes capping materials underneath a final restoration to seal the tooth after delivering cells into the canal system. Studying the immediate biocompatibility of materials with injected cells has been challenging. This study aimed to utilize a tube model to observe cell response directly and conveniently to the capping materials in a cell culture in vitro system. METHODS: A tapered plastic tube (14 mm in length, 2 mm diameter of the apex opening) to simulate root canal space was filled with fluorescence-labeled dental pulp cells mixed in fibrin gel and the larger end sealed with various capping materials including Mineral Trioxide Aggregate (MTA), Biodentine, hydroxyapatite-tricalcium phosphate (HA-TCP), composite, and glass ionomer. The tube was placed in wells of culture plates and incubated for various times in vitro and observed under the microscope for cell morphological changes. pH changes within the tube were monitored over time. RESULTS: Both fresh MTA and Biodentine caused adverse response to the cells in the tube. Only a few normally growing cells were observed at the apical end. Composite or glass ionomer appeared better tolerated by cells. HA-TCP mixed in fibrin gel demonstrated the highest compatibility with cells; however, using HA-TCP to separate cells from fresh MTA or Biodentine did not reduce the negative effect of these 2 calcium silicate cements. The pH increased within the tube may explain the observed toxicity. CONCLUSIONS: Using HA-TCP mixed in fibrin gel as a capping material appears highly biocompatible to cells while fresh MTA and Biodentine are not.
Citace poskytuje Crossref.org
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- $a Pilbauerová, Nela, $d 1990- $u Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Dentistry, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic; Department of Dentistry, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic $7 xx0214576
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- $a INTRODUCTION: Cell-based pulp regeneration utilizes capping materials underneath a final restoration to seal the tooth after delivering cells into the canal system. Studying the immediate biocompatibility of materials with injected cells has been challenging. This study aimed to utilize a tube model to observe cell response directly and conveniently to the capping materials in a cell culture in vitro system. METHODS: A tapered plastic tube (14 mm in length, 2 mm diameter of the apex opening) to simulate root canal space was filled with fluorescence-labeled dental pulp cells mixed in fibrin gel and the larger end sealed with various capping materials including Mineral Trioxide Aggregate (MTA), Biodentine, hydroxyapatite-tricalcium phosphate (HA-TCP), composite, and glass ionomer. The tube was placed in wells of culture plates and incubated for various times in vitro and observed under the microscope for cell morphological changes. pH changes within the tube were monitored over time. RESULTS: Both fresh MTA and Biodentine caused adverse response to the cells in the tube. Only a few normally growing cells were observed at the apical end. Composite or glass ionomer appeared better tolerated by cells. HA-TCP mixed in fibrin gel demonstrated the highest compatibility with cells; however, using HA-TCP to separate cells from fresh MTA or Biodentine did not reduce the negative effect of these 2 calcium silicate cements. The pH increased within the tube may explain the observed toxicity. CONCLUSIONS: Using HA-TCP mixed in fibrin gel as a capping material appears highly biocompatible to cells while fresh MTA and Biodentine are not.
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- $a Huang, George T-J $u Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Endodontics, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee; Department of Physiology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee. Electronic address: gtjhuang@uthsc.edu
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