Transport kinetics of 6-deoxy-D-glucose in Candida parapsilosis
Language English Country United States Media print
Document type Journal Article
PubMed
23984
DOI
10.1007/bf02876591
Knihovny.cz E-resources
- MeSH
- Aerobiosis MeSH
- Biological Transport, Active drug effects MeSH
- Amino Acids metabolism MeSH
- Candida metabolism MeSH
- Deoxy Sugars metabolism MeSH
- Deoxyglucose metabolism MeSH
- Dicyclohexylcarbodiimide pharmacology MeSH
- Dinitrophenols pharmacology MeSH
- Uranyl Nitrate pharmacology MeSH
- Iodoacetamide pharmacology MeSH
- Carbonyl Cyanide m-Chlorophenyl Hydrazone pharmacology MeSH
- Kinetics MeSH
- Hydrogen-Ion Concentration MeSH
- Carbohydrate Metabolism MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amino Acids MeSH
- Deoxy Sugars MeSH
- Deoxyglucose MeSH
- Dicyclohexylcarbodiimide MeSH
- Dinitrophenols MeSH
- Uranyl Nitrate MeSH
- Iodoacetamide MeSH
- Carbonyl Cyanide m-Chlorophenyl Hydrazone MeSH
The strictly aerobic yeast Candida parapsilosis transports the nonmetabolizable monosaccharide 6-deoxy-D-glucose by an active process (inhibition by 2.4-dinitrophenol and other uncouplers but not by iodoacetamide), the accumulation ratio decreasing with increasing substrate concentration. Measured accumulation ratios are in agreement with those predicted from kinetic constants for influx and efflux. Energy for transport is probably required in the translocation step. The maximum rate is temperature-dependent with a transition point at 21 degrees C. the accumulation ratio is not. The uptake is most active at pH 4.5--8.5. It appears not to involve stoichiometric proton symport. The transport system is shared by D-glucose, D-mannose, D-galactose and possibly maltose but not by fructose, sucrose or pentoses. The apparent half-life of the transport system was 3.5--4 h.
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J Theor Biol. 1975 Sep;53(1):125-44 PubMed
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Folia Microbiol (Praha). 1978;23(2):118-25 PubMed
Biochim Biophys Acta. 1965 Jul 22;102(2):410-22 PubMed
Biochim Biophys Acta. 1977 Nov 1;470(3):484-91 PubMed
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