Transport kinetics of 6-deoxy-D-glucose in Candida parapsilosis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
23984
DOI
10.1007/bf02876591
Knihovny.cz E-zdroje
- MeSH
- aerobióza MeSH
- aktivní transport účinky léků MeSH
- aminokyseliny metabolismus MeSH
- Candida metabolismus MeSH
- deoxycukry metabolismus MeSH
- deoxyglukosa metabolismus MeSH
- dicyklohexylkarbodiimid farmakologie MeSH
- dinitrofenoly farmakologie MeSH
- dusičnan uranylu farmakologie MeSH
- jodacetamid farmakologie MeSH
- karbonylkyanid-m-chlorfenylhydrazon farmakologie MeSH
- kinetika MeSH
- koncentrace vodíkových iontů MeSH
- metabolismus sacharidů MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aminokyseliny MeSH
- deoxycukry MeSH
- deoxyglukosa MeSH
- dicyklohexylkarbodiimid MeSH
- dinitrofenoly MeSH
- dusičnan uranylu MeSH
- jodacetamid MeSH
- karbonylkyanid-m-chlorfenylhydrazon MeSH
The strictly aerobic yeast Candida parapsilosis transports the nonmetabolizable monosaccharide 6-deoxy-D-glucose by an active process (inhibition by 2.4-dinitrophenol and other uncouplers but not by iodoacetamide), the accumulation ratio decreasing with increasing substrate concentration. Measured accumulation ratios are in agreement with those predicted from kinetic constants for influx and efflux. Energy for transport is probably required in the translocation step. The maximum rate is temperature-dependent with a transition point at 21 degrees C. the accumulation ratio is not. The uptake is most active at pH 4.5--8.5. It appears not to involve stoichiometric proton symport. The transport system is shared by D-glucose, D-mannose, D-galactose and possibly maltose but not by fructose, sucrose or pentoses. The apparent half-life of the transport system was 3.5--4 h.
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Coupling of secondary active transport with a deltamu-H+
