The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N'-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixture of squalene and its hydrosqualene derivatives and also properties of membrane-associated proteins were compared in wild-type and mutant cells. The impairment of the bioenergetic system in DCCD-resistant mutant was detectable in the membrane-protein profile; it was also accompanied by changes in proportions of squalene-hydrosqualenes.

• MeSH
• antibiotická rezistence MeSH
• buněčná membrána metabolismus   MeSH
• dicyklohexylkarbodiimid farmakologie   metabolismus   MeSH
• energetický metabolismus MeSH
• membránové proteiny genetika   metabolismus   MeSH
• Methanobacteriaceae genetika   metabolismus   účinky léků   MeSH
• mutace MeSH
• skvalen chemie   metabolismus   MeSH

• MeSH
• acetylcholin metabolismus   MeSH
• calcimycin farmakologie   MeSH
• dicyklohexylkarbodiimid farmakologie   MeSH
• synaptozomy metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH