Using incubation in the presence of 0.4 mM cycloheximide the half-lives of the principal membrane transport proteins in baker's yeast were found to be: more than 24 h for the constitutive glucose carrier, 2.2 h for the inducible galactose carrier, 1.2 h for the inducible maltose carrier and 0.8 h for the inducible alpha-methyl-D-glucoside carrier. The distinct nature of the two last-named carriers was thus supported. De-induction of the galactose carrier was enhanced in the presence of glucose plus cycloheximide but not of either substance alone. Chloramphenicol suppressed all effects of cycloheximide. In contrast to the enzymes of galactose metabolism, the induction of the glactose carrier was not under the control of a mitochondrial factor and took place in a rho-mutant. The system induced by maltose but not the one induced by alpha-methyl-D-glucoside was de-induced rapidly by the intervention of a cytoplasm-synthesized protein.

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Biochim Biophys Acta. 1969 Jul 30;184(2):299-305 PubMed

Folia Microbiol (Praha). 1974;19(6):479-88 PubMed

Mol Gen Genet. 1973;123(1):29-41 PubMed

J Bacteriol. 1963 Nov;86:966-70 PubMed

J Bacteriol. 1968 May;95(5):1727-31 PubMed

Biochim Biophys Acta. 1964 Mar 16;82:538-46 PubMed

Biochim Biophys Acta. 1961 Sep 2;52:176-83 PubMed

Biochim Biophys Acta. 1964 Mar 16;82:547-55 PubMed

Genetics. 1964 May;49:837-44 PubMed

Mol Gen Genet. 1971;110(2):110-7 PubMed

Folia Microbiol (Praha). 1968;13(1):12-9 PubMed

Biochim Biophys Acta. 1972 Aug 9;274(2):575-83 PubMed

Folia Microbiol (Praha). 1967;12(2):121-31 PubMed

J Bacteriol. 1968 Feb;95(2):603-11 PubMed

Folia Microbiol (Praha). 1972;17(5):353-6 PubMed

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