Factors affecting protein synthesis during biotin deficiency in Aspergillus nidulans
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
43277
DOI
10.1007/bf02927119
Knihovny.cz E-zdroje
- MeSH
- alanintransaminasa metabolismus MeSH
- aminokyseliny metabolismus MeSH
- aspartátaminotransferasy metabolismus MeSH
- Aspergillus nidulans metabolismus MeSH
- bezbuněčný systém MeSH
- biotin metabolismus MeSH
- DNA fungální biosyntéza MeSH
- fungální proteiny biosyntéza MeSH
- fungální RNA biosyntéza MeSH
- glutamátdehydrogenasa metabolismus MeSH
- glutaminsynthetasa metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alanintransaminasa MeSH
- aminokyseliny MeSH
- aspartátaminotransferasy MeSH
- biotin MeSH
- DNA fungální MeSH
- fungální proteiny MeSH
- fungální RNA MeSH
- glutamátdehydrogenasa MeSH
- glutaminsynthetasa MeSH
Biotin deficiency in Aspergillus nidulans resulted in a 70% increase of the protein content and increased levels of free and bound aspartate, glutamate, serine, leucine and methionine. Likewise, the activities of NADP+ glutamate dehydrogenase, NAD+ gluatmate dehydrogenase, aspartate aminotransferase and alanine aminotransferase were significantly increased. The total RNA content increased while the DNA content was unaffected. The rRNA/tRNA ratio remained higher in biotin-deficient cells. Supplementation of glutamate, aspartate, serine, leucine and methionine to the culture medium raised the rRNA/tRNA ratio, and the difference observed in the qualitative and the quantitative patterns of protein and dry cell mass between normal and biotin-deficient cultures was abolished.
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