Mapping of the chromosome of Mycobacterium phlei by means of mutagenesis of the replication point
Language English Country United States Media print
Document type Journal Article
PubMed
1248781
DOI
10.1007/bf02879001
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial MeSH
- Cell Division MeSH
- DNA, Bacterial biosynthesis MeSH
- Genes MeSH
- Leucine biosynthesis MeSH
- Chromosome Mapping * methods MeSH
- Methionine biosynthesis MeSH
- Methylnitronitrosoguanidine MeSH
- Mutation MeSH
- Mutagens MeSH
- Mycobacterium phlei * drug effects growth & development metabolism MeSH
- Mycobacterium * MeSH
- DNA Replication MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Leucine MeSH
- Methionine MeSH
- Methylnitronitrosoguanidine MeSH
- Mutagens MeSH
The aim of the present work was to construct a replication map of the chromosome of Mycobacterium phlei. The method of mutagenesis of the replication point by means of nitrosoguanidine was applied to synchronously multiplying populations. Back mutations and forward mutations were induced in auxotrophic mutants PA met and PA leu as well as in double auxotrophic mutants with methionine as the reference marker and the following order of replication of eleven genes on the chromosome was thus established: leu-Eth, Res-Stm, Cyk-pur-met, arg, Cyk-Bac-inl.
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Use of nalidixic acid for constructing the replication map of the Mycobacterium phlei chromosome
Mutagenic effect of n-methyl-N-nitrosourea on Mycobacterium phlei
The completion of the replication map of the Mycobacterium phlei chromosome
The replication map of the chromosome of Mycobacterium phlei
Genetic transfers in Mycobacteria
