It was the aim of the present work to construct the replication map of the chromosome of Mycobacterium phlei. The method of mutagenesis of the replication point by N-methyl-N-nitroso-N'-nitroguanidine in synchronously dividing populations and the method of analysis of gene frequency were applied. The order of replication of 19 genes on the chromosome was determined by means of induction of back mutations and forward mutations in auxotrophic mutants PA leu and PA met and in double auxotrophic mutants with methionine as a reference marker.

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Mycobacteria in the light of modern genetics development

Cell cycle in Mycobacterium phlei

Use of nalidixic acid for constructing the replication map of the Mycobacterium phlei chromosome

Mutagenic effect of n-methyl-N-nitrosourea on Mycobacterium phlei

The completion of the replication map of the Mycobacterium phlei chromosome