Radioprotection of mice by the bacterial extract Broncho-Vaxom: haemopoietic stem cells and survival enhancement
Language English Country Great Britain, England Media print
Document type Journal Article
- MeSH
- Adjuvants, Immunologic therapeutic use MeSH
- Bacteria * MeSH
- Cell Extracts * MeSH
- Radiation Injuries, Experimental prevention & control MeSH
- Hematopoietic Stem Cells radiation effects MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Radiation-Protective Agents therapeutic use MeSH
- Cell Survival radiation effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Broncho-Vaxom MeSH Browser
- Cell Extracts * MeSH
- Radiation-Protective Agents MeSH
Pretreatment of mice with 50-1000 micrograms of the bacterial extract Broncho-Vaxom (BV, free of endotoxin) before sublethal irradiation induced an increase in the number of endogenous haemopoietic stem cells (E-CFU). The degree of radioprotection was dependent on both the time of administration and the dose of BV. An optimal E-CFU survival was observed when 500 micrograms of BV was administered i.p. 24 h before irradiation. BV did not affect the day 9 CFU-S survival in the bone marrow directly after irradiation. However, 5, 9 and 12 days after irradiation, the number of day 9 CFU-S was almost 2-fold higher in the bone marrow of BV injected mice. Pretreatment with BV protected C57B1/6 mice in a dose-dependent manner from the lethal effect of ionizing radiation. A single dose (50, 100, 250, or 500 micrograms) of bacterial lysate injected i.p. 24 h before 9.5 Gy gamma-rays (LD100/21) protected 16%, 25%, 80%, and 94% of C57B1/6 mice, respectively. The dose reduction factor in the case when the BV (500 micrograms per mouse) was administered at that time was 1.18 (95% CL 1.12, 1.25).
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