Permeability factor, cytotoxicity and serotyping of Pseudomonas aeruginosa strains
Language English Country United States Media print
Document type Journal Article
PubMed
1493904
DOI
10.1007/bf02815663
Knihovny.cz E-resources
- MeSH
- Cell Death MeSH
- Capillary Permeability MeSH
- Rabbits MeSH
- Humans MeSH
- Neoplasms microbiology MeSH
- Pseudomonas aeruginosa classification isolation & purification pathogenicity MeSH
- Serotyping MeSH
- Organ Transplantation MeSH
- Vero Cells MeSH
- Ear, External microbiology MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
We analyzed in detail the permeability and cytotoxic activity as well as the serotypes of 127 Pseudomonas aeruginosa strains. Sixty-seven strains were isolated from immunocompromised patients (51 from patients with tumors and 16 from patients after transplantation) and 60 strains were isolated from patient's ears. Culture filtrates of strains isolated from patients after transplantation were responsible for the highest part of permeability reactions corresponding to an intermediate toxin production (68.8%) (categories 2 and 3) and culture filtrates of strains isolated from patients with tumors caused the highest percentage of permeability reactions corresponding to a strong toxin production. Culture filtrates of strains isolated from ears of patients were responsible for the highest percentage of negative permeability reactions (15%). With positive permeability reaction size (categories 2-6) increased also the percentage of cytotoxicity as well as the intensity of morphological changes on Vero cells after 1 and 2 d. We did not observe any relationship between a particular permeability reaction category and the most frequent serotypes (O4, O6) or nontypable strains of the tested groups.
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