Yeast ADP/ATP carrier (AAC) proteins exhibit similar enzymatic properties but their deletion produces different phenotypes
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
1618335
DOI
10.1016/0014-5793(92)80637-v
PII: 0014-5793(92)80637-V
Knihovny.cz E-zdroje
- MeSH
- adenosindifosfát metabolismus MeSH
- fenotyp MeSH
- kinetika MeSH
- mitochondriální ADP/ATP-translokasy genetika metabolismus MeSH
- mitochondrie metabolismus MeSH
- mutační analýza DNA MeSH
- Saccharomyces cerevisiae genetika MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- adenosindifosfát MeSH
- mitochondriální ADP/ATP-translokasy MeSH
Saccharomyces cerevisiae strains expressing a single type of ADP/ATP carrier (AAC) protein were prepared from a mutant in which all AAC genes were disrupted, by transformation with plasmids containing a chosen AAC gene. As demonstrated by measurements of [14C]ADP specific binding and transport, all three translocator proteins, AAC1, AAC2 and AAC3 when present in the mitochondrial membrane, exhibited similar translocation properties. The disruption of some AAC genes, however, resulted in phenotypes indicating that the function of these proteins in whole cells can be quite different. Specifically, we found that the disruption of AAC1 gene, but not AAC2 and AAC3, resulted in a change in colony phenotype.
Citace poskytuje Crossref.org
