The ADP/ATP carrier and its relationship to oxidative phosphorylation in ancestral protist trypanosoma brucei
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25616281
PubMed Central
PMC4346568
DOI
10.1128/ec.00238-14
PII: EC.00238-14
Knihovny.cz E-zdroje
- MeSH
- mitochondriální ADP/ATP-translokasy chemie genetika metabolismus MeSH
- molekulární evoluce * MeSH
- oxidativní fosforylace * MeSH
- protozoální proteiny chemie genetika metabolismus MeSH
- Trypanosoma brucei brucei genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mitochondriální ADP/ATP-translokasy MeSH
- protozoální proteiny MeSH
The highly conserved ADP/ATP carrier (AAC) is a key energetic link between the mitochondrial (mt) and cytosolic compartments of all aerobic eukaryotic cells, as it exchanges the ATP generated inside the organelle for the cytosolic ADP. Trypanosoma brucei, a parasitic protist of medical and veterinary importance, possesses a single functional AAC protein (TbAAC) that is related to the human and yeast ADP/ATP carriers. However, unlike previous studies performed with these model organisms, this study showed that TbAAC is most likely not a stable component of either the respiratory supercomplex III+IV or the ATP synthasome but rather functions as a physically separate entity in this highly diverged eukaryote. Therefore, TbAAC RNA interference (RNAi) ablation in the insect stage of T. brucei does not impair the activity or arrangement of the respiratory chain complexes. Nevertheless, RNAi silencing of TbAAC caused a severe growth defect that coincides with a significant reduction of mt ATP synthesis by both substrate and oxidative phosphorylation. Furthermore, TbAAC downregulation resulted in a decreased level of cytosolic ATP, a higher mt membrane potential, an elevated amount of reactive oxygen species, and a reduced consumption of oxygen in the mitochondria. Interestingly, while TbAAC has previously been demonstrated to serve as the sole ADP/ATP carrier for ADP influx into the mitochondria, our data suggest that a second carrier for ATP influx may be present and active in the T. brucei mitochondrion. Overall, this study provides more insight into the delicate balance of the functional relationship between TbAAC and the oxidative phosphorylation (OXPHOS) pathway in an early diverged eukaryote.
Biology Center Institute of Parasitology Czech Academy of Sciences České Budějovice Czech Republic
Department of Biochemistry Faculty of Natural Sciences Comenius University Bratislava Slovakia
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Trypanosoma brucei TbIF1 inhibits the essential F1-ATPase in the infectious form of the parasite
