One-step preparation of Hageman factor fragment concentrate
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
1643388
DOI
10.1002/bmc.1130060408
Knihovny.cz E-resources
- MeSH
- Enzyme Activation MeSH
- Chromatography, Liquid MeSH
- Factor XII chemistry isolation & purification MeSH
- Blood MeSH
- Humans MeSH
- Peptide Fragments chemistry isolation & purification MeSH
- Prekallikrein metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Factor XII MeSH
- Peptide Fragments MeSH
- Prekallikrein MeSH
The Hageman factor fragment (HFf) concentrate was prepared from acetone and dextran sulphate activated human plasma by chromatography on a CM-Sephadex column. The preparation was free of the main kallikrein inhibitors--Cl-inactivator, alpha 2-macroglobulin, antithrombin III and alpha 1-antitrypsin. The HFf concentrate can serve as an activator of prekallikrein in patient plasma.
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