Low content of mitochondrial ATPase in brown adipose tissue is the result of post-transcriptional regulation
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
1661683
DOI
10.1016/0014-5793(91)80666-q
PII: 0014-5793(91)80666-Q
Knihovny.cz E-zdroje
- MeSH
- adenosintrifosfatasy genetika metabolismus MeSH
- exprese genu MeSH
- genetická transkripce * MeSH
- hnědá tuková tkáň enzymologie MeSH
- játra enzymologie MeSH
- messenger RNA genetika metabolismus MeSH
- mitochondrie enzymologie MeSH
- mozek enzymologie MeSH
- myokard enzymologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- nízká teplota MeSH
- northern blotting MeSH
- proteosyntéza MeSH
- respirační komplex IV genetika metabolismus MeSH
- svaly enzymologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adenosintrifosfatasy MeSH
- messenger RNA MeSH
- respirační komplex IV MeSH
The mRNA levels of ATPase beta, ATPase 6, cytochrome oxidase (COX) VIb and COX I subunits were found to be 2.4-13.8-fold higher in brown adipose tissue (BAT) than in heart, skeletal muscle, brain and liver of mice. The comparison with tissue contents of ATPase and COX revealed that the selective, 5-11-fold reduction of ATPase in BAT is not caused by decreased transcription of ATPase genes. Likewise, the ATPase beta and COX VIb mRNA levels in cultured brown adipocytes were also not influenced by norepinephrine, which activated the expression of the UCP gene by two orders of magnitude. The results indicate that the biosynthesis of mitochondrial ATPase in BAT is post-transcriptionally regulated.
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