Low content of mitochondrial ATPase in brown adipose tissue is the result of post-transcriptional regulation
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
1661683
DOI
10.1016/0014-5793(91)80666-q
PII: 0014-5793(91)80666-Q
Knihovny.cz E-resources
- MeSH
- Adenosine Triphosphatases genetics metabolism MeSH
- Gene Expression MeSH
- Transcription, Genetic * MeSH
- Adipose Tissue, Brown enzymology MeSH
- Liver enzymology MeSH
- RNA, Messenger genetics metabolism MeSH
- Mitochondria enzymology MeSH
- Brain enzymology MeSH
- Myocardium enzymology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Cold Temperature MeSH
- Blotting, Northern MeSH
- Protein Biosynthesis MeSH
- Electron Transport Complex IV genetics metabolism MeSH
- Muscles enzymology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine Triphosphatases MeSH
- RNA, Messenger MeSH
- Electron Transport Complex IV MeSH
The mRNA levels of ATPase beta, ATPase 6, cytochrome oxidase (COX) VIb and COX I subunits were found to be 2.4-13.8-fold higher in brown adipose tissue (BAT) than in heart, skeletal muscle, brain and liver of mice. The comparison with tissue contents of ATPase and COX revealed that the selective, 5-11-fold reduction of ATPase in BAT is not caused by decreased transcription of ATPase genes. Likewise, the ATPase beta and COX VIb mRNA levels in cultured brown adipocytes were also not influenced by norepinephrine, which activated the expression of the UCP gene by two orders of magnitude. The results indicate that the biosynthesis of mitochondrial ATPase in BAT is post-transcriptionally regulated.
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