Stabilization of a miniderivative of the broad-host-range IncW plasmid pSa by insertion of plasmid R1 parB region
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
1668749
DOI
10.1007/bf02814352
Knihovny.cz E-zdroje
- MeSH
- antibiotická rezistence MeSH
- Brevibacterium genetika MeSH
- druhová specificita MeSH
- Escherichia coli genetika MeSH
- genetické markery MeSH
- R-plasmidy genetika MeSH
- rekombinace genetická MeSH
- transpozibilní elementy DNA * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- genetické markery MeSH
- transpozibilní elementy DNA * MeSH
The plasmid vector pEM100 (13.5 kb) constructed from pGV1106, a miniderivative of the broad-host-range IncW pSa plasmid, and the pAM330 plasmid of Brevibacterium lactofermentum is not stably maintained in Escherichia coli host cells under nonselective growth conditions. By insertion of a 0.9 kb DNA fragment containing the parB locus (responsible for the maintenance of plasmid R1 in E. coli cells) to plasmid pEM100, plasmid pEM110 was prepared which is maintained in a population of E. coli cells growing without a selection pressure very stably.
Zobrazit více v PubMed
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