Hybrid plasmid pIM138 was constructed by insertion of a chromosomal fragment with the threonine operon from Escherichia coli into the pBR322 vector. Molar mass of pIM138 was 2.8 Mg/mol. Heteroduplexes between pBR322 vector and pIM138 hybrid DNA molecules were prepared. The hybrid plasmid shows a high stability against the curing effect of rifampicin and clorobiocin in E. coli SK1590 thr host.

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Production of threonine by Brevibacterium flavum containing threonine biosynthesis genes from Escherichia coli

Stabilization of a miniderivative of the broad-host-range IncW plasmid pSa by insertion of plasmid R1 parB region

Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria

Plasmid pIMI38--the pBR322 derivative with increased stability in E. coli cells