Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria
Language English Country United States Media print
Document type Journal Article
PubMed
2148164
DOI
10.1007/bf02821278
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial genetics MeSH
- Transformation, Bacterial MeSH
- Brevibacterium genetics MeSH
- Corynebacterium drug effects genetics MeSH
- DNA, Bacterial analysis genetics MeSH
- Escherichia coli genetics MeSH
- Genetic Vectors * MeSH
- Cloning, Molecular MeSH
- R Factors * MeSH
- Gene Expression Regulation, Bacterial MeSH
- Restriction Mapping MeSH
- Spectinomycin pharmacology MeSH
- Streptomycin pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Spectinomycin MeSH
- Streptomycin MeSH
A new shuttle vector pCEM500 replicating in Escherichia coli and in Brevibacterium flavum was constructed. It carries two antibiotic resistance determinants (Kmr/Gmr from plasmid pSa of Gram-negative bacteria and Smr/Spr from plasmid pCG4 of Corynebacterium glutamicum) which are efficiently expressed in both hosts and can be inactivated by insertion of DNA fragments into the unique restriction endonuclease sites located within them. This vector was found to be stably maintained in B. flavum and can be used for transfer of the cloned genes into this amino-acid-producing coryneform bacterium.
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