Production of threonine by Brevibacterium flavum containing threonine biosynthesis genes from Escherichia coli

. 1993 ; 38 (5) : 355-9.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid08262444

Genes of the threonine operon of Escherichia coli were used for the construction of a Brevibacterium flavum strain excreting threonine. Using the shuttle vector pCEM300 and a newly constructed shuttle vector pEC71 (7.1 kb, Kmr/Nmr), various plasmids carrying E. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hydroxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment of B. flavum carrying these recombinant plasmids. A mutant of B. flavum CCM 351 carrying the cloned genes thrA and thrB accumulated 12 g/L of threonine after 48 h of cultivation.

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Folia Microbiol (Praha). 1990;35(4):273-7 PubMed

Mol Gen Genet. 1987 Mar;206(3):361-7 PubMed

Genetika. 1990 Apr;26(4):648-56 PubMed

Nucleic Acids Res. 1987 Dec 23;15(24):10598 PubMed

Folia Microbiol (Praha). 1968;13(2):125-8 PubMed

Gene. 1987;56(2-3):309-12 PubMed

J Bacteriol. 1975 Dec;124(3):1137-41 PubMed

FEMS Microbiol Lett. 1989 Dec;53(3):299-303 PubMed

Gene. 1991 Oct 30;107(1):53-9 PubMed

Mol Microbiol. 1988 Jan;2(1):63-72 PubMed

J Bacteriol. 1984 Jul;159(1):306-11 PubMed

Biochim Biophys Acta. 1963 Jan 8;67:16-30 PubMed

Folia Microbiol (Praha). 1983;28(6):441-5 PubMed

Appl Microbiol Biotechnol. 1991 Feb;34(5):617-22 PubMed

Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PubMed

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