Production of threonine by Brevibacterium flavum containing threonine biosynthesis genes from Escherichia coli
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8262444
DOI
10.1007/bf02898754
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial genetics MeSH
- Brevibacterium enzymology genetics metabolism MeSH
- Escherichia coli genetics MeSH
- Gene Expression physiology MeSH
- Phosphotransferases (Alcohol Group Acceptor) metabolism MeSH
- Genetic Vectors MeSH
- Homoserine Dehydrogenase metabolism MeSH
- Lysine biosynthesis MeSH
- Operon MeSH
- Plasmids MeSH
- Threonine biosynthesis genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phosphotransferases (Alcohol Group Acceptor) MeSH
- Homoserine Dehydrogenase MeSH
- homoserine kinase MeSH Browser
- Lysine MeSH
- Threonine MeSH
Genes of the threonine operon of Escherichia coli were used for the construction of a Brevibacterium flavum strain excreting threonine. Using the shuttle vector pCEM300 and a newly constructed shuttle vector pEC71 (7.1 kb, Kmr/Nmr), various plasmids carrying E. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hydroxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment of B. flavum carrying these recombinant plasmids. A mutant of B. flavum CCM 351 carrying the cloned genes thrA and thrB accumulated 12 g/L of threonine after 48 h of cultivation.
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