Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2148164
DOI
10.1007/bf02821278
Knihovny.cz E-zdroje
- MeSH
- antibiotická rezistence genetika MeSH
- bakteriální transformace MeSH
- Brevibacterium genetika MeSH
- Corynebacterium účinky léků genetika MeSH
- DNA bakterií analýza genetika MeSH
- Escherichia coli genetika MeSH
- genetické vektory * MeSH
- klonování DNA MeSH
- R-plasmidy * MeSH
- regulace genové exprese u bakterií MeSH
- restrikční mapování MeSH
- spektinomycin farmakologie MeSH
- streptomycin farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA bakterií MeSH
- spektinomycin MeSH
- streptomycin MeSH
A new shuttle vector pCEM500 replicating in Escherichia coli and in Brevibacterium flavum was constructed. It carries two antibiotic resistance determinants (Kmr/Gmr from plasmid pSa of Gram-negative bacteria and Smr/Spr from plasmid pCG4 of Corynebacterium glutamicum) which are efficiently expressed in both hosts and can be inactivated by insertion of DNA fragments into the unique restriction endonuclease sites located within them. This vector was found to be stably maintained in B. flavum and can be used for transfer of the cloned genes into this amino-acid-producing coryneform bacterium.
Zobrazit více v PubMed
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